Skip to main content
Help the Python Software Foundation raise $60,000 USD by December 31st!  Building the PSF Q4 Fundraiser

Geneset Network Analysis

Project description

# PyGNA: a Python framework for geneset network analysis

![GitHub tag (latest by date)]( [![Anaconda-Server Badge](]( ![Build]( ![Release](

PyGNA is a framework for statistical network analysis of high-throughput experiments. It can be used both as a standalone command line application or it can be used as API to develop custom analyses.

For an overview of PyGNA functionalities check the infographic below or dive into our [Getting started](#getting-started) tour.


## Installation

The easiest and fastest way to install pygna using conda:

$ conda install -c stracquadaniolab -c bioconda -c conda-forge pygna

Alternatively you can install it through pip:

$ pip install pygna

We also provide a docker image installation with the latest version of PyGNA. It can be easily executed from the command line from DockerHub:

$ docker run stracquadaniolab/pygna/pygna:latest

or GitHub Packages:

$ docker run

which will show the PyGNA command line help.

## Getting started

A typical pygna analysis consists of 3 steps:

  1. Generate the RWR and SP matrices for the network you are using ( once they are generated, you won’t need to repeat the same step again)
  2. Make sure that the input genesets are in the right format. If a network uses entrez ID, and your file is in HUGO symbols, use the pygna utility for the name conversion.
  3. Run the analysis you are interested into.
  4. Once you have the output tables, you can choose to visualize one or more plots.

Otherwise you can check our [snakemake workflow]( for the full geneset analysis; our workflow contains sample data that you can use to familiarize with our software.

The examples below show some basic analysis that can be carried out with pygna.

### Example 1: Running pygna GNT analysis

Running pygna on this input as follows:

$ cd ./your-path/min-working-example/

$ pygna build-rwr-diffusion barabasi.interactome.tsv –output-file interactome_RWR.hdf5

$ pygna test-topology-rwr barabasi.interactome.tsv disgenet_cancer_groups_subset.gmt interactome_RWR.hdf5 table_topology_rwr.csv –number-of-permutations 1000 –cores 4

$ pygna paint-datasets-stats table_topology_rwr.csv barplot_rwr.pdf

You can look at the plot of the results in the barplot_rwr.pdf file, and the corresponding table in table_topology_rwr.csv.

### Example 2: Running pygna GNA analysis

$ cd ./your-path/min-working-example/

skip this step if the matrix is already computed

$ pygna build-RWR-diffusion barabasi.interactome.tsv –output-file interactome_RWR.hdf5

The association analysis is run N x M times (N number of genesets, M number of pathways), we use only 50 permutations in this example to avoid long computations; however, the recommended value is 1000.

$ pygna test-association-rwr barabasi.interactome.tsv disgenet_cancer_groups_subset.gmt interactome_RWR.hdf5 table_association_rwr.csv -B disgenet_cancer_groups_subset.gmt –keep –number-of-permutations 100 –cores 4

If you don’t include the –results-figure flag at the comparison step, plot the matrix as follows

$ pygna paint-comparison-matrix table_association_rwr.csv heatmap_association_rwr.png –rwr –annotate

The -k flag, keeps the -B geneset and permutes only on the set A.

If setname B is not passed, the analysis is run between each couple of setnames in the geneset.

$ pygna test-association-rwr barabasi.interactome.tsv disgenet_cancer_groups_subset.gmt interactome_RWR.hdf5 table_within_comparison_rwr.csv –number-of-permutations 100 –cores 4

$ pygna paint-comparison-matrix table_within_comparison_rwr.csv heatmap_within_comparison_rwr.png –rwr –single-geneset

You can look at the plot of the results in the heatmap_within_comparison_rwr.png file, and the corresponding table in table_within_comparison_rwr.csv.

## Documentation

The official documentation for pygna can be found on [readthedocs](

## Authors

## Citation

A unified framework for geneset network analysis. Viola Fanfani, Giovanni Stracquadanio. bioRxiv 699926; doi:

## Issues

Please post an issue to report a bug or request new features.

Project details

Download files

Download the file for your platform. If you're not sure which to choose, learn more about installing packages.

Files for pygna, version 3.3.0
Filename, size File type Python version Upload date Hashes
Filename, size pygna-3.3.0-py3-none-any.whl (56.0 kB) File type Wheel Python version py3 Upload date Hashes View
Filename, size pygna-3.3.0.tar.gz (48.0 kB) File type Source Python version None Upload date Hashes View

Supported by

Pingdom Pingdom Monitoring Google Google Object Storage and Download Analytics Sentry Sentry Error logging AWS AWS Cloud computing DataDog DataDog Monitoring Fastly Fastly CDN DigiCert DigiCert EV certificate StatusPage StatusPage Status page