Sequence Elements Enrichment Analysis (SEER), python implementation
pyseer –phenotypes phenotypes.tsv –kmers kmers.gz –distances structure.tsv –min-af 0.01 –max-af 0.99 –cpu 15 –filter-pvalue 1E-8
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Kmers-based GWAS analysis is particularly well suited for bacterial samples, given their high genetic variability. This approach has been implemented by [Lees, Vehkala et al.](https://www.nature.com/articles/ncomms12797), in the form of the [SEER](https://github.com/johnlees/seer) software.
The reimplementation presented here should be consistent with the current version of the C++ seer (though we do not guarantee this for all possible cases).
In this version, as well as all the original features, many new features (input types, association models and output parsing) have been implemented. See the [documentation](http://pyseer.readthedocs.io/) and [paper](https://doi.org/10.1093/bioinformatics/bty539) for full details.
Lees, John A., Galardini, M., et al. "pyseer: a comprehensive tool for microbial pangenome-wide association studies". Bioinformatics 34:4310–4312 (2018).
Lees, John A., et al. "Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes." Nature communications 7:12797 (2016).
Full documentation is available at [readthedocs](http://pyseer.readthedocs.io/).
You can also build the docs locally (requires sphinx) by typing:
cd docs/ make html
Between parenthesis the versions the script was tested against:
- python 3+ (3.6.6)
- numpy (1.15.2)
- scipy (1.1.0)
- pandas (0.23.4)
- scikit-learn (0.20.0)
- statsmodels (0.9.0)
- pysam (0.15.1)
- glmnet_python (commit 946b65c)
- DendroPy (4.4.0)
If you would like to use the scree_plot_pyseer script you will also need to have matplotlib installed. If you would like to use the scripts to map and annotate kmers, you will also need bwa, bedtools, bedops and pybedtools installed.
The easiest way to install pyseer and its dependencies is through conda:
conda install pyseer
If you need conda, download [miniconda](https://conda.io/miniconda.html) and add the necessary channels:
conda config --add channels defaults conda config --add channels bioconda conda config --add channels conda-forge
pyseer can also be installed through pip:
python -m pip install pyseer
If you want multithreading make sure that you are using a version 3 python interpreter:
`python3 -m pip install pyseer`
If you want the next pre-release, just clone/download this repository and run:
Copyright 2017 EMBL-European Bioinformatics Institute
Licensed under the Apache License, Version 2.0 (the “License”); you may not use this file except in compliance with the License. You may obtain a copy of the License at
Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an “AS IS” BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.
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