🐍✂️ PytrimAl 
Cython bindings and Python interface to trimAl, a tool for automated alignment trimming. Now with SIMD!
⚠️ This package is based on the release candidate of trimAl 2.0, and results may not be consistent across versions or with the trimAl 1.4 results.
🗺️ Overview
PytrimAl is a Python module that provides bindings to trimAl using Cython. It implements a user-friendly, Pythonic interface to use one of the different trimming methods from trimAl and access results directly. It interacts with the trimAl internals, which has the following advantages:
- single dependency: PytrimAl is distributed as a Python package, so you can add it as a dependency to your project, and stop worrying about the trimAl binary being present on the end-user machine.
- no intermediate files: Everything happens in memory, in a Python object
you control, so you don't have to invoke the trimAl CLI using a
sub-process and temporary files.
Alignmentobjects can be created directly from Python code. - friendly interface: The different trimming methods are implement as Python classes that can be configured independently.
- error management: Errors occuring in trimAl are converted transparently into Python exceptions, including an informative error message.
- better performance: PytrimAl uses SIMD instructions to compute statistics like pairwise sequence similarity. This makes the whole trimming process much faster for alignment with a large number of sequences, at the expense of slightly higher memory consumption.
📋 Roadmap
The following features are available or considered for implementation:
- automatic trimming: Support for trimming alignments using one of the automatic heuristics implemented in trimAl.
- manual trimming: Support for trimming alignments using manually defined conservation and gap thresholds for each residue position.
- overlap trimming: Trimming sequences using residue and sequence overlaps to exclude regions with minimal conservation.
- representative trimming: Select only representative sequences from the alignment, either using a fixed number, or a maximum identity threshold.
- alignment loading from disk: Load an alignment from disk given a filename.
- alignment loading from a file-like object: Load an alignment from a Python file object instead of a file on the local filesystem.
- aligment creation from Python: Create an alignment from a collection of sequences stored in Python strings.
- alignment formatting to disk: Write an alignment to a file given a filename in one of the supported file formats.
- alignment formatting to a file-like object: Write an alignment to a file-like object in one of the supported file formats.
- reverse-translation: Back-translate a protein alignment to align the sequences in genomic space.
- alternative similarity matrix: Specify an alternative similarity matrix for the alignment (instead of BLOSUM62).
- similarity matrix creation: Create a similarity matrix from scratch from Python code.
- windows for manual methods: Use a sliding window for computing statistics in manual methods.
🔧 Installing
PytrimAl is available for all modern versions (3.6+), with no external dependencies.
It can be installed directly from PyPI, which hosts some pre-built wheels for the x86-64 architecture (Linux/OSX) and the Aarch64 architecture (Linux only), as well as the code required to compile from source with Cython:
$ pip install pytrimal
Otherwise, pytrimal is also available as a Bioconda package:
$ conda install -c bioconda pytrimal
💡 Example
Let's load an Alignment from a file on the disk, and use the strictplus
method to trim it, before printing the TrimmedAlignment as a Clustal block:
from pytrimal import Alignment, AutomaticTrimmer
ali = Alignment.load("pytrimal/tests/data/example.001.AA.clw")
trimmer = AutomaticTrimmer(method="strictplus")
trimmed = trimmer.trim(ali)
for name, seq in zip(trimmed.names, trimmed.sequences):
print(name.decode().rjust(6), seq)
This should output the following:
Sp8 GIVLVWLFPWNGLQIHMMGII
Sp10 VIMLEWFFAWLGLEINMMVII
Sp26 GLFLAAANAWLGLEINMMAQI
Sp6 GIYLSWYLAWLGLEINMMAII
Sp17 GFLLTWFQLWQGLDLNKMPVF
Sp33 GLHMAWFQAWGGLEINKQAIL
You can then use the
dump
method to write the trimmed alignment to a file or file-like
object. For instance, save the results in
PIR format
to a file named example.trimmed.pir:
trimmed.dump("example.trimmed.pir", format="pir")
🧶 Thread-safety
Trimmer objects are thread-safe, and the trim method is re-entrant.
This means you can batch-process alignments in parallel using a
ThreadPool
with a single trimmer object:
import glob
import multiprocessing.pool
from pytrimal import Alignment, AutomaticTrimmer
trimmer = AutomaticTrimmer()
alignments = map(Alignment.load, glob.iglob("pytrimal/tests/data/*.fasta"))
with multiprocessing.pool.ThreadPool() as pool:
trimmed_alignments = pool.map(trimmer.trim, alignments)
⏱️ Benchmarks
Benchmarks were run on a i7-10710U CPU
@ 1.10GHz, using a single core to time the computation of several statistics,
on a variable number of sequences from
example.014.AA.EggNOG.COG0591.fasta,
an alignment of 3583 sequences and 7287 columns.
Each graph measures the computation time of a single trimAl statistic (see the Statistics page of the online documentation for more information.)
The None curve shows the time using the internal trimAl 2.0 code,
the Generic curve shows a generic C implementation with some more
optimizations, and the SSE curve shows the time spent using a dedicated
class with SIMD
implementations of the statistic computation.
💭 Feedback
⚠️ Issue Tracker
Found a bug ? Have an enhancement request ? Head over to the GitHub issue tracker if you need to report or ask something. If you are filing in on a bug, please include as much information as you can about the issue, and try to recreate the same bug in a simple, easily reproducible situation.
🏗️ Contributing
Contributions are more than welcome! See
CONTRIBUTING.md
for more details.
📋 Changelog
This project adheres to Semantic Versioning and provides a changelog in the Keep a Changelog format.
⚖️ License
This library is provided under the GNU General Public License v3.0.
trimAl is developed by the trimAl team and is distributed under the
terms of the GPLv3 as well. See vendor/trimal/LICENSE for more information.
This project is in no way not affiliated, sponsored, or otherwise endorsed by the trimAl authors. It was developed by Martin Larralde during his PhD project at the European Molecular Biology Laboratory in the Zeller team.
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Release files for pytrimal 0.8.5
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