Fast Leica LIF file reader written in python
readlif README file
The readlif package was developed to be a fast, python only, reader for Leica Lif files. This is tested in Python 3.6 through 3.9.
The basic premise is to read an image from a Lif file into a Pillow object. The only additional requirement for this package is Pillow>=7.2.0.
This code is inspired by the Open Microscopy Bio-Formats project.
Auto-generated documentation is available here.
This package is available on pypi, so you can install it with pip
pip install readlif
Alternatively, clone the git repo and install with setuptools
python setup.py install
There may be an issue where a truncated 16-bit file will load incorrectly, however this is not tested. If you have an example file, open an issue!
There is not support for FlipX, FlipY and SwapXY metadata. If you need this, please open an issue!
Truncated images are returned as blank images.
There is currently no support for returning arbitrary planes from
display_dims. This is in progress.
12- and 16-bit images
As of 0.3.0,
reaflif will now support images with bit depth greater than 8.
However, note that while some images will be returned as a 16 bit array, they may
actually 10 or 12 bit images. It is not simple to easily convert these without
the potential of losing data, so a new
bit_depth attribute has been added
LifImage to indicate the bit depth of each channel in the image.
It is up to the user to decide how, or if, to convert these. There is an upscaling example below.
Everything in this package is numbered starting from 0, which is not consistent with how things like ImageJ operate.
The basic object is the LifFile object.
from readlif.reader import LifFile new = LifFile('./path/to/file.lif')
This object contains a few methods to access the images contained within the Lif file. All images, in a folder or not, will be accessible sequentially from the
# Access a specific image directly img_0 = new.get_image(0) # Create a list of images using a generator img_list = [i for i in new.get_iter_image()]
LifImage object has a few methods to access the specific two-dimensional frame contained in the image, where z is the z position, t is the timepoint, and c is the channel.
# Access a specific item img_0.get_frame(z=0, t=0, c=0) # Iterate over different items frame_list = [i for i in img_0.get_iter_t(c=0, z=0)] z_list = [i for i in img_0.get_iter_z(t=0, c=0)] channel_list = [i for i in img_0.get_iter_c(t=0, z=0)]
The two dimensional images returned by these methods are Pillow objects, so the applicable methods (
.show()) will work with them.
If it is necessary to scale a 12-bit image to the full 16-bit range, it is possible to do this with numpy.
import numpy as np # Assumes all channels have the same bit depth scale_factor = (16 - img_0.bit_depth) ** 2 frame = img_0.get_frame(z=0, t=0, c=0) img_array = np.uint16(np.array(frame) * scale_factor) Image.fromarray(img_array).show()
This has only been tested on Lif files that were generated with Leica LAS X and Leica LAS AF. There will likely be files that will not work with this software. In that case, please open an issue on github!
- Added support for updated LasX with additional data in the Lif file
- Changed the way file names are assigned, now includes folder path
- This fix is intended to clarify imaging sets with many duplicate names
- Fixed bug where the time and image scale were calculated incorrectly
- Clarified scaling documentation
- Update readme
- Added support for non-XY images with
get_plane(). See docs for usage.
- Note: Reading arbitrary planes (i.e. an XZ plane of an XY image) is not yet supported.
- Bugfix: Fix error in mosaic parsing.
PosXmetadata was incorrectly read from
- Bugfix: switch from
- Added support for loading files from buffers
- Thans to PR from @JacksonMaxfield
- Fixed critical documentaiton error:
LifImage.scaleis in px/µm, not px/nm for X and Y dimensions
- Added support for tiled images
mwas added as a new dimension (for tiled images)
(FieldX, FieldY, PosX, PosY)
- Under the hood changes
LifImage.dimsis now a named tuple for clearer code
- Other things
- Prettier outputs for
- Switch to github CI
- Prettier outputs for
- Added error message for tiled images, pending feature addition
- Added support for 16-bit images, increased minimum Pillow version to 7.2.0.
bit_depthis a tuple of intigers descibing the bit depth for each channel in the image.
- Thanks to @DirkRemmers for providing the example file.
- Changed type from
- Added python 3.9 to build testing
ZeroDivisionErrorwhen the Z-dimension is defined, but has a length of 0. Clarified an error message. Added fix for truncated files.
LifImage.scalenow returns px/nm conversions
- Style changes
- Initial release
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