RNF framework for NGS: simulation of reads, evaluation of mappers, conversion of RNF-compliant data.
Project description
Read Naming Format is a generic format for assigning read names with encoded information about original positions. RNFtools is an associated software package which can:
simulate RNF-compliant reads using a wide class of integrated read simulators (Art, DwgSim, Mason, WgSim, etc.), either from a single genome (i.e., a whole genome sequencing) or multiple genomes (i.e., a metagenomic simulation);
evaluate mappers using RNF reads;
convert non-RNF simulated reads to RNF (e.g., from SAM format);
transform genomic coordinates of RNF reads between different coordinate systems (using chain LiftOver format).
Links
Web of the project: http://karel-brinda.github.io/rnftools/
RNF specification: http://karel-brinda.github.io/rnf-spec/
Documentation: http://rnftools.rtfd.org
Installation: http://rnftools.readthedocs.org/en/latest/tutorial/00_installation.html
Examples of usage: http://github.com/karel-brinda/rnftools/tree/master/examples/01_tutorial
Publication: http://dx.doi.org/10.1093/bioinformatics/btv524
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