scbl-utils 1.15

A set of command-line utilities that facilitate data processing at the Single Cell Biology Lab at the Jackson Laboratory.

scbl-utils

A set of command-line utilities that facilitates data processing in the Single Cell Biology Lab at the Jackson Laboratory.

Top-level Usage

scbl-utils [OPTIONS] COMMAND [ARGS]...

Example Usage

scbl-utils samplesheet-from-gdrive /path/to/fastq_dir /path/to/another/fastq_dir /path/to/a_dir/of/fastq_dirs/*

Top-level Options

• --config-dir, -c: Configuration directory containing files necessary for script to run. (default: /sc/service/etc/.config/scbl-utils)

Commands

• samplesheet-from-gdrive

  Pull data from Google Drive and generate a yml samplesheet to be used as input for the nf-tenx pipeline.

  Usage

  scbl-utils samplesheet-from-gdrive [OPTIONS] FASTQDIRS
  

  Options

  • --outsheet, -o: File path to save the resulting samplesheet. [default: samplesheet.yml]
  • --reference-path-as-str, -s: If possible, write the reference_path field of the outputted samplesheet as a string rather than a list of strings. This enables compatability with the current nf-tenx pipeline and wll be deprecated in the future as the pipeline is updated.

  Requirements

  For the script to work, the config-dir (as defined here) must contain a directory called google-drive. In {config-dir}/google-drive, 3 files must exist:

  • trackingsheet-spec.yml: a specification that instructs the script on how to read data from the Google Sheet being used as the "sample tracking sheet". The specification must contain the following keys:

    • id: the Google Spreadsheet ID, found in https://docs.google.com/spreadsheets/d/`spreadsheet_id`/

    • sheets: a mapping of the ID of each worksheet (docs.google.com/spreadsheets/d/spreadsheet_id/edit#gid=worksheet_id) within the spreadsheet to another mapping of information about that sheet. The keys of this dict must contain:

      • columns: yet another mapping, this time mapping the column names in the sheet to how they should be renamed in the script. The union of all of the values of these mappings should minimally be {10x_platform, sample_name, is_nuclei, libraries, project, species, n_cells, slide, area, tag_id}, and libraries must exist in the sheets where join == true to join the sheets.
      • header_row: The index of the header row (0-based), which contains the column names
      • join: a bool indicating whether to join this sheet (along the columns) to the other sheets in the spreadsheet. Useful for spreadsheets with multiple sheets, but not every sheet shares the same index, meaning they are not necessarily joinable

    • platform_to_lib_type: another mapping, this time mapping the name of a 10X platform to the library type

      Example trackingsheet-spec.yml:

      id: <spreadsheet_id>
      sheets:
          0:
              columns:
                  10X Platform: 10x_platform
                  Customer ID: sample_name
                  Is Nuclei: is_nuclei
                  Sample Name (SCBID): libraries
                  SCBL Project: project
                  Species: species
                  Targeted Cell Recovery: n_cells
              header_row: 2
              join: true
          2:
              columns:
                  Sample Name (SCBID): libraries
                  Serial Number GEX Slide: slide
              header_row: 2
              join: true
          4:
              columns:
                  Sample Name (SCBID): libraries
                  Position on Slide: area
              header_row: 2
              join: true
          5:
              columns:
                  Customer ID: sub_sample_name
                  Pool ID: sample_name
                  SCID: libraries
                  Tag ID: tag_id
                  Tissue/Cell Type: description
              header_row: 0
              join: false
      platform_to_lib_type:
          3' RNA: Gene Expression
          3' RNA-HT: Gene Expression  
          5' RNA: Gene Expression
          5' RNA-HT: Gene Expression
          5' VDJ: Immune Profiling
          ATAC: Chromatin Accessibility
          ATAC v2: Chromatin Accessibility
          Automated RNA: Gene Expression
          Cell Surface: Antibody Capture
          CellPlex: Multiplexing Capture
          Flex: Gene Expression
          HTO: Multiplexing Capture
          LMO: Multiplexing Capture
          Multiome ATAC: Chromatin Accessibility
          Multiome RNA: Gene Expression
          RNA: Gene Expression
          RNA-HT: Gene Expression
          Visium CytAssist FFPE: CytAssist Gene Expression
          Visium FF: Spatial Gene Expression
          Visium FFPE: Spatial Gene Expression
      

  • metricssheet-spec.yml: a specification that instructs the script on how to read metrics sheets from Google Drive. This is useful for automated assignment of processing tool, tool version, and reference genome, as the script looks for old metrics spreadsheets within the same "SCBL Project" to assign these values when possible. The necessary keys are similar to trackingsheet-spec.yml:

    • dir_id: the ID of the Google Drive folder where delivered metrics are stored. Found in https://drive.google.com/drive/folders/`folderID`

    • header_row: the header row of the metrics sheets. This assumes that all sheets within all spreadsheets in the metrics delivery folder have the same header row

    • columns: just like trackingsheet-spec.yml, this is a mapping of the column names as they appear in the spreadsheets to how they should be named in the script. The union of these columns should minimally be {project, tool, tool_version, reference, libraries}. Because different metrics sheets have the libraries column as a different name, it may be necessary to add key-value pairs to this if the script throws a pandas error along the lines of KeyError: '{key}'. Because the script uses this mapping to determine what columns in the spreadsheet should go into a pandas.DataFrame, it will throw an error if it hasn't been informed that a certain column in the spreadsheet is really the project column, for example.

      Example metricssheet-spec.yml

      dir_id: <dir_id>
      header_row: 0
      columns:
          SCBL Project: project
          Processing Tool: tool
          Processing Tool Version: tool_version
          Processing Reference: reference
          Library ID(s): libraries
          Sample ID: libraries
      

  • service-account.json: A json file that stores credentials for a service account associated with a Google Cloud Project. This shouldn't be an issue, but if the script is throwing Google Drive login errors, this file might need to be regenerated and put in {config-dir}/google-drive. See the gspread documentation for instructions.

  Adding New Tool Versions

  Because scbl-utils samplesheet-from-gdrive is designed to be used in conjunction with the nf-tenx pipeline, it queries the container collection the pipeline pulls from to know what versions of tools are available. If you have the required permissions, you can simply add a new definition file to the registry, ensuring that the file name you add follows the convention of the other definition files. Note that you won't be able to access the registry if you're not connected to JAX WiFi.