scipion-em-aretomo 3.5.1

Plugin to use AreTomo program within the Scipion framework

This plugin provides a wrapper for AreTomo program.

Installation

You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:

1. Stable version

scipion installp -p scipion-em-aretomo

2. Developer’s version

   • download repository

   git clone -b devel https://github.com/scipion-em/scipion-em-aretomo.git

   • install

   scipion installp -p /path/to/scipion-em-aretomo --devel

• AreTomo binaries will be installed automatically with the plugin, but you can also link an existing installation.

• Default installation path assumed is software/em/aretomo-1.3.3, if you want to change it, set ARETOMO_HOME in scipion.conf file to the folder where the AreTomo is installed.

• Depending on your CUDA version this plugin will guess the right default binary from AreTomo_1.3.3_CudaXY_11212022 (X is for cuda major version, Y for the minor). You can always set a different one by explicitly setting ARETOMO_BIN variable.

• If you need to use CUDA different from the one used during Scipion installation (defined by CUDA_LIB), you can add ARETOMO_CUDA_LIB variable to the config file. Various binaries can be downloaded from the official UCSF website.

To check the installation, simply run the following Scipion test:

scipion test aretomo.tests.test_protocols_aretomo.TestAreTomo

Licensing

AreTomo is free for academic use only. For commercial use, please contact David Agard or Yifan Cheng for licensing:

• [e-mail]

• [e-mail]

Supported versions

1.0.6, 1.0.8, 1.0.10, 1.0.12, 1.1.0, 1.1.1, 1.2.0, 1.2.5, 1.3.0, 1.3.3

Protocols

• tilt-series align and reconstruct

Detailed manual can be found in software/em/aretomo-1.3.3/bin/AreTomoManual_1.3.0_09292022.pdf

References

• AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction. Shawn Zheng, Georg Wolff, Garrett Greenan, Zhen Chen, Frank G. A. Faas, Montserrat Bárcena, Abraham J. Koster, Yifan Cheng, David Agard. JSB vol. 6, 2022, 100068.