Localrec ready to use in scipion.
Project description
Localrec Scipion plugin
Cryogenic electron microscopy (cryoEM) can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. We have developed a general method for the localized three-dimensional reconstruction of such subunits earlier (Ilca et al 2015 Nature Commun).
In this project we implement Localized Reconstruction as a plugin (Locarec) for Scipion.
If Localized Reconstruction is useful in your work, please cite:
Ilca SL, Kotecha A, Sun X, Poranen MM, Stuart DI & Huiskonen JT (2015). Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes. Nat Commun 6, 8843. doi:10.1038/ncomms9843
Abrishami V, Ilca SL, Gomez-Blanco J, Rissanen I, de la Rosa-Trevín JM, Reddy VS, Carazo JM, Huiskonen JT (2020). Localized reconstruction in Scipion expedites the analysis of symmetry mismatches in cryo-EM data. Prog. Biophys. Mol. Biol. doi:10.1016/j.pbiomolbio.2020.05.004.
Install this plugin
You will need to use 3.0.0 version of Scipion or later to run these protocols.
Stable version
Either use the following command
scipion3 installp -p scipion-em-localrec
or
Install plugin through the plugin manager GUI by launching Scipion and following Configuration >> Plugins
Developer’s version
Download repository:
git clone https://github.com/scipion-em/scipion-em-localrec.git
Install:
scipion3 installp -p path_to_scipion-em-localrec --devel
Test the plugin:
scipion3 test localrec.tests.test_protocol_localized_reconstruction
Protocols
The following protocols have been implemented so far
localrec - define subparticles: Determines the coordinates, orientations and defocus values of the sub-particles.
localrec - filter subparticles: Excludes unwanted sub-particles based on alignment information.
localrec - extract subparticles: Extracts sub-particle images from each particle image.
localrec - set origin to subvolume: Fits a sub-volume back to it’s original volume (larger volume).
localrec - particles subset by subparticles: makes a subset of particles for which there is at least one sub-particle.
localrec - stitch subvolumes: Assembles a full volume from the sub-volumes generated by localized reconstruction.
Tests
scipion test localrec.tests.test_protocol_localized_reconstruction
Buildbot status
Status devel version:
Status production version:
Acknowledgements
The authors acknowledge funding from the European Research Council under the European Union’s Horizon 2020 research and innovation programme (649053).
Project details
Release history Release notifications | RSS feed
Download files
Download the file for your platform. If you're not sure which to choose, learn more about installing packages.
Source Distribution
Built Distribution
Hashes for scipion-em-localrec-3.0.4.tar.gz
Algorithm | Hash digest | |
---|---|---|
SHA256 | d20fb1ee9cdd71227fe87c9ad36c86753aa1ac8d3e3cb6294bbb7c5343fee48d |
|
MD5 | 86f8715bba4e18c13d1d033fe24dc537 |
|
BLAKE2b-256 | 6c8213d973f85fd931ae87bd333e8bfc9b7526a024918f36caa9ce7f4a2906e0 |
Hashes for scipion_em_localrec-3.0.4-py3-none-any.whl
Algorithm | Hash digest | |
---|---|---|
SHA256 | 2a92944118a5f90c26db80a4ea299b46dce16304c0c78bb174026854683a0e58 |
|
MD5 | 4cde3bb77b74254641ee61d7d5f75f63 |
|
BLAKE2b-256 | 1eb7579e9b99fa3ac6631ca5a753d8fb2f8cb008a8c75cadb581101427f6a720 |