Plugin to use NovaCTF in Scipion
Project description
This plugin provides wrappers for several programs of NovaCTF software suite.
Installation
You will need to use 3.0+ version of Scipion to be able to run these protocols. To install the plugin, you have two options:
Stable version
scipion installp -p scipion-em-novactf
Developer’s version
download repository
git clone -b devel https://github.com/scipion-em/scipion-em-novactf.git
install
scipion installp -p /path/to/scipion-em-novactf --devel
NovaCTF binaries will be downloaded and installed automatically with the plugin, but you can also link an existing installation. Default installation path assumed is software/em/novactf-master, if you want to change it, set NOVACTF_HOME in scipion.conf file to the folder where the NovaCTF is installed.
To check the installation, simply run the test below:
scipion3 tests novactf.tests.test_protocols_novactf.TestNovaCtfReconstructionWorkflow
Supported versions
master (8 Nov 2018)
Protocols
compute defocus array
3D CTF correction and reconstruction
References
Beata Turoňová and Florian K.M. Schur and William Wan and John A.G. Briggs. Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å. Journal of Structural Biology 199 (3), p. 187-195, 2017. doi: https://doi.org/10.1016/j.jsb.2017.07.007
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