scipion-em-sphire 1.2.7

Sphire ready to use in scipion.

Plugin to use Sphire programs within the Scipion framework

So far we have implemented:

• crYOLO particle picker (current version: 1.3.3)

crYOLO installation

• Please follow installation instructions from: http://sphire.mpg.de/wiki/doku.php?id=downloads:cryolo_1&redirect=1 in order to install:

  • crYOLO (last tested version: 1.3.3) (should download a file called cryolo-X.Y.Z.tar.gz)

  • crYOLO’s boxmanager (last tested version: 1.1.0). (should download a file called cryoloBM-X.Y.Z.tar.gz) Note: the BoxManager is not strictly necessary for using the Scipion protocols.

• If you want to use the generic model, please download also the General PhosaursNet model. (latest at the time of writing: ftp://ftp.gwdg.de/pub/misc/sphire/crYOLO-GENERAL-MODELS/gmodel_phosnet_20190314.h5, but better to check the Sphire page for the latest one)

The installation should be pretty easy if you have already a conda installation.

conda create -n cryolo -c anaconda python=3.6 pyqt=5 cudnn=7.1.2 numpy==1.14.5
pip install cryolo-X.Y.Z.tar.gz
pip install cryoloBM-X.Y.Z.tar.gz

Scipion crYOLO Configuration

Then, we need to define some environment variables to specify how to load the cryolo environment and where is the general model. We can define the variables in the .bashrc file or in ~/.config/scipion/scipion.conf:

CRYOLO_ENV_ACTIVATION = '. /path/to/anaconda/etc/profile.d/conda.sh; conda activate cryolo'
CRYOLO_GENERIC_MODEL = path/to/the/downloaded/General_PhosaursNet_model

Install Scipion crYOLO Plugin

scipion installp -p scipion-em-sphire

OR

• through the plugin manager GUI by launching Scipion and following Configuration >> Plugins

OR

scipion python -m pip install scipion-em-sphire

If you are developing the plugin, other useful options are:

scipion installp -p local/path/to/scipion-em-sphire --devel

OR

git clone [e-mail]:scipion-em/scipion-em-sphire
export PYTHONPATH=$PYTHONPATH:$PWD/scipion-em-sphire

Running crYOLO tests

To check that everything is properly installed and configured, you might want to run some tests:

scipion test --grep cryolo --run