seqcluster 0.99.13

Small RNA-seq pipeline

seqcluster
---------

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small RNA analysis from NGS data

.. image:: https://travis-ci.org/lpantano/seqcluster.png?branch=master
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:target: https://pypi.python.org/pypi/seqcluster


Cite
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A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome.
Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5.
PMID: 21976421

installation
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`seqcluster-helper`_ provides
a python framework to run a whole pipeline for small RNA (miRNA + others).

Install first bcbio-nextgen and cutadapter after install conda if you want a isolate env::

wget http://repo.continuum.io/miniconda/Miniconda-latest-Linux-x86_64.sh
bash Miniconda-latest-Linux-x86_64.sh -b -p ~/install/seqcluster/anaconda
PATH = ~/install/seqcluster/anaconda/bin:$PATH
conda install pip
conda install -c https://conda.binstar.org/bcbio bcbio-nextgen
pip install cutadapt
pip install matplotlib
pip install -U cython


Remember to add the new python into your path every time you want to user seqcluster.
If you already have `conda` in your system, just type::

conda install -c https://conda.binstar.org/bcbio bcbio-nextgen

If you need to install bedtools, samtools and star, follow these steps::

git clone https://github.com/Homebrew/linuxbrew.git ~/install/seqcluster/linuxbrew
cd ~/install/seqcluster/linuxbrew/bin
ln -s `which gcc gcc-4.4`
PATH = ~/install/seqcluster/linuxbrew/bin:$PATH
brew tab homebrew/science
brew tab chapmanb/homebre-cbl
brew install bedtools
brew install samtools
brew install star-rna


Then you can get seqcluster::

pip install seqcluster

or the developement version::

git clone https://github.com/lpantano/seqcluster
cd seqcluster
python setup.py install


.. _seqcluster-helper: https://github.com/lpantano/seqcluster-helper/blob/master/README.md


quick start
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Complete tutorial here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

report
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Seqcluster creates html report that looks like `this`_. That is a table of all cluster detected, and you
can go into each of them and get a complete `description`_ with profile expression figures, annotation details and
sequences counts for each sample.

.. _this: https://rawgit.com/lpantano/seqcluster/master/data/examples_report/html/index.html
.. _description: https://rawgit.com/lpantano/seqcluster/master/data/examples_report/html/1/maps.html