Skip to main content

This is is the fastqc pipeline from the Sequana <https://sequana.readthedocs.org> projet

Overview:

Runs fastqc and multiqc on a set of Sequencing data to produce control quality reports

Input:

A set of FastQ files (paired or single-end) compressed or not

Output:

an HTML file index.html (multiqc) and tree.html (individual fastqc report)

Status:

production

Usage

sequana_pipelines_fastqc --help
sequana_pipelines_fastqc --input-directory DATAPATH --run-mode local
sequana_pipelines_fastqc --input-directory DATAPATH --run-mode slurm

This creates a directory fastq. You just need to execute the pipeline:

cd fastqc
sh fastqc.sh  # for a local run

This launch a snakemake pipeline. If you are familiar with snakemake, you can retrieve the fastqc.rules and config.yaml files and then execute the pipeline yourself with specific parameters:

snakemake -s fastqc.rules --cores 4 --stats stats.txt

Or use sequanix interface.

Requirements

This pipelines requires the following executable(s):

  • fastqc

https://raw.githubusercontent.com/sequana/sequana_fastqc/master/sequana_pipelines/fastqc/dag.png

Details

This pipeline runs fastqc in parallel on the input fastq files (paired or not) and then execute multiqc. A brief sequana summary report is also produced.

Rules and configuration details

Here is the latest documented configuration file to be used with the pipeline. Each rule used in the pipeline may have a section in the configuration file.

Download files

Download the file for your platform. If you're not sure which to choose, learn more about installing packages.

Source Distribution

sequana_fastqc-0.8.1.tar.gz (54.5 kB view details)

Uploaded Source

File details

Details for the file sequana_fastqc-0.8.1.tar.gz.

File metadata

  • Download URL: sequana_fastqc-0.8.1.tar.gz
  • Upload date:
  • Size: 54.5 kB
  • Tags: Source
  • Uploaded using Trusted Publishing? No
  • Uploaded via: twine/1.15.0 pkginfo/1.5.0.1 requests/2.22.0 setuptools/40.4.3 requests-toolbelt/0.9.1 tqdm/4.39.0 CPython/3.5.5

File hashes

Hashes for sequana_fastqc-0.8.1.tar.gz
Algorithm Hash digest
SHA256 008070ea11359e240b08761a8db58284c44515e770d02f30b33b3242db884c50
MD5 3a9d360df895cc53f763d452f8625a6c
BLAKE2b-256 8c8e163e194e6fe41de84e27b14948645c1fa6a9b60d0df2fd0d9f7a2bfd4cfc

See more details on using hashes here.

Supported by

AWS Cloud computing and Security Sponsor Datadog Monitoring Depot Continuous Integration Fastly CDN Google Download Analytics Sentry Error logging StatusPage Status page