SeqUDAS: Sequence Upload and Data Archiving System
Project description
SeqUDAS: Sequence Upload and Data Archiving System
Overview
Modern DNA sequencing machines generate several gigabytes (GB) of data per run. Organizing and archiving this data presents a challenge for small labs. We present a Sequence Upload and Data Archiving System (SeqUDAS) that aims to ease the task of maintaining a sequence data repository through process automation and an intuitive web interface.
Features
- Automated upload and storage of sequence data to a central storage server.
- Data validation with MD5 checksums for data integrity assurance
- Illumina modules are incorpated to parse metrics binaries and generate a report similar to Illumina SAV.
- FASTQC and MultiQC workflows are included to perform QC analysis automatically.
- A taxonomic report will be generated based on Kraken report
- Archival information, QC results and taxonomic report can be viewed through a mobile-friendly web interface
- Pass sequence data along to another remote server via API (IRIDA)
Architecture
SeqUDAS consists of three components:
- Data manager: Installed on a PC directly attached to an illumina sequencing machine.
- Data analyzer: Installed on a server to run the data analysis jobs.
- Web Application: Installed on a web server to provide account management and report viewing.
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