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SeqUDAS: Sequence Upload and Data Archiving System

Project description

SeqUDAS: Sequence Upload and Data Archiving System

Overview

Modern DNA sequencing machines generate several gigabytes (GB) of data per run. Organizing and archiving this data presents a challenge for small labs. We present a Sequence Upload and Data Archiving System (SeqUDAS) that aims to ease the task of maintaining a sequence data repository through process automation and an intuitive web interface.

Features

  • Automated upload and storage of sequence data to a central storage server.
  • Data validation with MD5 checksums for data integrity assurance
  • Illumina modules are incorpated to parse metrics binaries and generate a report similar to Illumina SAV.
  • FASTQC and MultiQC workflows are included to perform QC analysis automatically.
  • A taxonomic report will be generated based on Kraken report
  • Archival information, QC results and taxonomic report can be viewed through a mobile-friendly web interface
  • Pass sequence data along to another remote server via API (IRIDA)

Architecture

SeqUDAS consists of three components:

  • Data manager: Installed on a PC directly attached to an illumina sequencing machine.
  • Data analyzer: Installed on a server to run the data analysis jobs.
  • Web Application: Installed on a web server to provide account management and report viewing.

Project details


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1.0.0

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