sequdas-client 1.0.0

SeqUDAS: Sequence Upload and Data Archiving System

SeqUDAS: Sequence Upload and Data Archiving System

Overview

Modern DNA sequencing machines generate several gigabytes (GB) of data per run. Organizing and archiving this data presents a challenge for small labs. We present a Sequence Upload and Data Archiving System (SeqUDAS) that aims to ease the task of maintaining a sequence data repository through process automation and an intuitive web interface.

Features

• Automated upload and storage of sequence data to a central storage server.
• Data validation with MD5 checksums for data integrity assurance
• Illumina modules are incorpated to parse metrics binaries and generate a report similar to Illumina SAV.
• FASTQC and MultiQC workflows are included to perform QC analysis automatically.
• A taxonomic report will be generated based on Kraken report
• Archival information, QC results and taxonomic report can be viewed through a mobile-friendly web interface
• Pass sequence data along to another remote server via API (IRIDA)

Architecture

SeqUDAS consists of three components:

• Data manager: Installed on a PC directly attached to an illumina sequencing machine.
• Data analyzer: Installed on a server to run the data analysis jobs.
• Web Application: Installed on a web server to provide account management and report viewing.