SigProfilerClusters 1.1.2

SigProfilerClusters tool

SigProfilerClusters

Tool for analyzing the inter-mutational distances between SNV-SNV and INDEL-INDEL mutations. Tool separates mutations into clustered and non-clustered groups on a sample-dependent basis and subclassifies all SNVs into a set category of clustered event: i) DBS; ii) MBS; iii) omikli; and iv) kataegis. Indels are not subclassifed.

This tool was previously under the project name of SigProfilerHotSpots, but has been renamed to SigProfilerClusters. For all instructions below, SigProfilerClusters may be interchanged with SigProfilerHotSpots if the older version of the tools is being used.

INTRODUCTION

The purpose of this document is to provide a guide for using the SigProfilerClusters framework. An extensive Wiki page detailing the usage of this tool can be found at https://osf.io/qpmzw/wiki/home/.

PREREQUISITES

The framework is written in PYTHON, and uses additional SigProfiler packages:

• PYTHON version 3.8 or newer
• SigProfilerMatrixGenerator (https://github.com/AlexandrovLab/SigProfilerMatrixGenerator)
• SigProfilerSimulator (https://github.com/AlexandrovLab/SigProfilerSimulator)

Please visit their respective GitHub pages for detailed installation and usage instructions.

QUICK START GUIDE

This section will guide you through the minimum steps required to perform clustered analysis:

1a. Install the python package using pip (current package): pip install SigProfilerClusters

1b. Install the python package using pip (deprecated version): pip install SigProfilerHotSpots

Install your desired reference genome from the command line/terminal as follows (available reference genomes are: GRCh37, GRCh38, mm9, and mm10):

$ python
>> from SigProfilerMatrixGenerator import install as genInstall
>> genInstall.install('GRCh37', rsync=False, bash=True)

This will install the human 37 assembly as a reference genome. You may install as many genomes as you wish. If you have a firewall on your server, you may need to install rsync and use the rsync=True parameter. Similarly, if you do not have bash, use bash=False.

2. Place your vcf files in your desired output folder. It is recommended that you name this folder based on your project's name. Before you can analyze clustered mutations, you need to generate a background model for each of your samples. To do this, generate a minimum of 100 simulations for your project (see SigProfilerSimulator for a detailed list of parameters):

>>from SigProfilerSimulator import SigProfilerSimulator as sigSim
>>sigSim.SigProfilerSimulator(project, project_path, genome, contexts=["96"], simulations=100, chrom_based=True)

3. Now the original mutations can be partitioned into clustered and non-clustered sets using the required parameters below:

>> from SigProfilerClusters import SigProfilerClusters as hp
>> hp.analysis(project, genome, contexts, simContext, input_path)

See below for a detailed list of available parameters

4. The partitioned vcf files are placed under [project_path]/ouput/clustered/ and [project_path]/ouput/nonClustered/. You can visualize the results by looking at the IMD plots available under [project_path]/ouput/plots/.

AVAILABLE PARAMETERS

Required:
        project:			[string] Unique name for the given project
        genome:			[string] Reference genome to use. Must be installed using SigProfilerMatrixGenerator
        contexts:			[string] Mutation context for measuring IMD (e.g. "6", "96", "1536", etc,)
        simContext: 		[list of strings] Mutations context that was used for generating the background model (e.g ["6144"] or ["96"])
        input_path:			[string] Path to the given project

	Optional:
        analysis:	 		[string] Desired analysis pipeline. By default output_type='all'. Other options include "subClassify" and "hotspot". 
        sortSims:			[boolean] Option to sort the simulated files if they have already been sorted. By default sortSims=True to ensure accurate results. The files must be sorted for accurate results. 
        interdistance:			[string] The mutation types to calculate IMDs between - Use only when performing analysis of indels (default='ID').
        calculateIMD:		[boolean] Parameter to calculate the IMDs. This will save time if you need to rerun the subclassification step only (default=True).
        max_cpu:			[integer] Change the number of allocated CPUs. By default all CPUs are used
        subClassify:		[boolean] Subclassify the clustered mutations. Requires that VAF scores are available in TCGA or Sanger format. By default subClassify=False. See VAF Format below for more details. 
        plotIMDfigure:	[boolean] Parameter that generates IMD and mutational spectra plots for each sample (default=True).
        plotRainfall		[boolean] Parameter that generates rainfall plots for each sample using the subclassification of clustered events (default=True).
        
        The following parameters are used if the subClassify argument is True:
        includedVAFs:	[boolean] Parameter that informs the tool of the inclusion of VAFs in the dataset (default=True)
        includedCCFs:   [boolean] Parameter that informs the tool of the inclusion of CCFs in the dataset (default=True). If CCFs are used, set includedVAFs=False.
        sanger:			[boolean] The input files are from Sanger. By default sanger=True
        TCGA:			[boolean] The input files are from TCGA. By default TCGA=False
        windowSize:		[integer] Window size for calculating mutation density in the rainfall plots. By default windowSize=10000000
        correction		[boolean] Optional parameter to perform a genome-wide mutational density correction (boolean; default=False)
        probability     [boolean] Optional parameter to calculate the probability of observing each clustered event within the localized region of the genome. These values are saved into the [project_path]/output/clustered/ directories. See OSF wiki page for more details.

VAF Format

SigProfilerClusters uses the VAF recorded in the input files to subclassify clustered mutations when subclassify=True and includedVAFs=True. However, depending on the VCF file, the VAF may be recorded in a different format. Below are the accepted formats corresponding to the correct settings to use for this tool.

If you are not using VCFs as input files, VAFs cannot be used in the subclassification step. Therefore, to subclassify clusters using other input file types set subclassify=True and includedVAFs=False.

If your VAF is recorded in the 8th column of your VCF as VCF=xx, set TCGA=True and sanger=False.

If your VAF is recorded in the 8th column of your VCF as AF=xx, set standardVC=True, TCGA=False, and sanger=False.

If your VAF is recorded in the 11th column of your VCF as the last number of the colon delimited values, set sanger=True and TCGA=False.

If your VCFs have no recorded VAFs set includedVAFs=False. This will run SigProfilerClusters, subclassify clusters based on just the calculated IMD (provided that you set subclassify=True).

CCF Format Alternatively, SigProfilerClusters accepts cancer cell fraction (CCF) estimates in place of VAFs, when includedCCFs=True and includedVAFs=False to correct for copy number amplifications. To use CCFs, one must add the CCF values in the last column of each row within each VCF file (tab separated columns).

Output Format All mutations are categorized into either clustered or non-clustered mutations. Each are saved under their respective directories within VCF files ([project_path]/output/clustered/ or [project_path]/output/nonClustered/; respectively). The clustered folder contains the partition of clustered mutations, which have been subclassified into one of four categories (five if using VAFs/CCFs). Within each subclass subfolder, a single VCF file is saved for each sample. All visualizations including rainfall plots are found within the output directory ([project_oath]/output/plots/). Please refer to the Output page on the OSF wiki page for more details on all available output including available plots (https://osf.io/qpmzw/wiki/4.%20Output/).

LOG FILES

All errors and progress checkpoints are saved into SigProfilerClusters_[project][genome].err and SigProfilerClusters[project]_[genome].out, respectively. For all errors, please email the error and progress log files to the primary contact under CONTACT INFORMATION.

Example Files Two examples are provided under examples/. These directories include all expected output except for the simulations folder to reduce memory size (please see the OSF page for more details: https://osf.io/qpmzw/wiki/4.%20Output/).

CITATIONS

Erik N Bergstrom, Mousumy Kundu, Noura Tbeileh, Ludmil B Alexandrov. bioRxiv 2022.02.11.480117; doi: https://doi.org/10.1101/2022.02.11.480117

COPYRIGHT

Copyright (c) 2023, Erik Bergstrom [Alexandrov Lab] All rights reserved.

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.

Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

CONTACT INFORMATION

Please address any queries or bug reports to Erik Bergstrom at [e-mail] or Mousumy Kundu at [e-mail]