tax2peptide 0.0.2

tax2peptide creates based on given taxon IDs and a reference database a taxon specific database in fasta format.

tax2peptide

tax2Peptide creates based on the given taxon IDs and a reference database a taxon specific database in fasta format. This taxon specific database contains according to the selected options all fasta entries of:

• the given taxon IDs and their descendant taxon IDs in the phylogenetic tree
• the given taxon IDs (option --no_descendants)
• the given taxon IDs adapted to specified level up in the phylogenetic tree and their descendant taxon IDs (option --level)
• the given taxon IDs and their descendant taxon IDs in the phylogenetic tree until level species, taxonIDs with lower level are not included (option --species)

Databases from which the matching entries are read out are the NCBI non-redundant peptide database, swissprot, uniprot or trembl database. Also user-defined databases are possible as long as the header of the database contains taxonIDs in form of "OX=NUMBER" or contains NCBI/uniprot accession numbers. Using uncompressed databases would speed up the program considerably.

Getting Started

Tax2Peptide is a python3 command line tool. It can be installed as pypi-package or as conda-package.

Prerequisites

Python3

needed packages: tqdm, biopython, wget

pip install tqdm, biopython, wget

Installing

pip install tax2peptide

Deployment

Tax2Peptide is a command line tool and starts with:

python3 -m tax2peptide [options]

Options:

-i --input TaxID input file: tabular file containing a column of NCBI taxon IDs. Columns tab separated.

-c --column The column (zero-based) in the tabular file that contains Taxon IDs. Default = 0.

-t --taxon NCBI taxon ID/s for database extraction. Multiple taxonIDs seperated by space.

-d --database Database choice for analysis or for download. Choices: ncbi, uniprot, tremble, swissprot.

-p --path Path to folder with all needed databases: taxdump.tar.gz (for all databases), prot.accession2taxid or prot.accession2taxid.gz and pdb.accession2taxid.gz (for ncbi databases). Optional: peptide_database named: nr/nr.gz or uniprot_trembl.fasta/uniprot_trembl.fasta.gz or uniprot_sprot.fasta/uniprot_sprot.fasta.gz or uniprot.fasta./uniprot.fasta.gz

-o --out File name and direction of the result taxon specified peptide database. Default = /taxon_specified_db_DATE/taxon_database.fasta

-n --dbname Database name and direction. If database is in other folder than --path or have a different name.

-l --level Hierarchy level up in anchestral tree. Choices: species, section, genus, tribe, subfamily, family, superfamily, order, superorder, class, phylum, kingdom, superkingdom

-r --non_redundant Make the final database non redundant in regard to sequences, headers are concatenated.

-z --no_descendants Select peptide database only by given taxon IDs, descendant taxons are excluded.

-s --species Select peptide database only until taxonomic level "species", descendants from species are excluded.

-u --threads Number of threads for using multiprocessing. Default = number of cores.

Dependencies:

Needed databases for NCBI reference database:

• protaccession2tax.gz / protaccession2tax
• pdbaccession2tax.gz
• taxdump.tar.gz
• nr.gz / nr

Needed databases for uniprot/swissprot/trembl reference database:

• taxdump.tar.gz
• uniprot.fasta.gz / uniprot.fasta / uniprot_sprot.fasta.gz / uniprot_sprot.fasta / uniprot_trembl.fasta.gz / uniprot_trembl.fasta

All database files should be downloaded the same day and stored in the same folder.

Databases

All should be downloaded at the same date as the peptide database to ensure successful accession matching. The database can be downloaded manually or downloaded by Tax2Peptide with option --database {ncbi, uniprot, trembl, swissprot}, file integrity would be md5-checked.

database name	description	source	adress
NCBI	non redundant peptide database	NCBI	ftp://ftp.ncbi.nlm.nih.gov/blast/db/FASTA/nr.gz
Swissprot	curated peptide database	Uniprot	ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_sprot.fasta.gz
Trembl	peptide database	Uniprot	ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_trembl.fasta.gz
Uniprot	concatenated swissprot and trembl database	Uniprot
prot.accession2taxid	contain links between accession IDs and taxonomic lineage (taxon IDs)	NCBI	ftp.ncbi.nlm.nih.gov/pub/taxonomy/accession2taxid/prot.accession2taxid.gz
pdb.accession2taxid	contain links between accession IDs and taxonomic lineage (taxon IDs)	NCBI	ftp.ncbi.nlm.nih.gov/pub/taxonomy/accession2taxid/pdb.accession2taxid.gz
taxdump	tar-gz-compressed taxdump file containing information about the phylogenetic lineage and links between taxIDs and scientific names etc.	NCBI	ftp://ftp.ncbi.nlm.nih.gov/pub/taxonomy/taxdump.tar.gz

Usage with download:

All needed databases will be downloaded to specified path (option --path). If not specified a folder with name databases_DATE will be used as default.

Examples of usage:

python3 -m tax2peptide -d uniprot -i path/to/input/taxon_ID_file  -> new Folder databases_DATE with: taxdump.tar.gz, uniprot.fasta

python3 -m tax2peptide -i path/to/input/taxon_ID_file  -> new Folder databases_DATE with: taxdump.tar.gz, uniprot.fasta

python3 -m tax2peptide -d ncbi -p path/to/my_new_databases -i path/to/input/taxon_ID_file  -> new Folder/used Folder my_new_databases with: protaccession2tax.gz, pdbaccession2tax.gz, taxdump.tar.gz, nr.gz

Usage if all database files are already downloaded:

• option --path determines folder with all needed databases (optional --dbname

• at least one taxon ID or taxon ID input file must be provided Path will be checked for all required database files and missing databases will be downloaded Examples of usage:

python3 -m tax2peptide -p path/to/folder -n path/to/reference_database -t 11111 22222 -o path/my_taxon_specified_database.fasta

python3 -m tax2peptide -p path/to/folder -n path/ to/ uniprot.fasta -t 11111 22222 -i path/to/input

python3 -m tax2peptide -d ncbi -p path/to/folder -i path/to/input

python3 -m tax2peptide -d uniprot -p path/to/folder -i path/to/input -o path/to/user_specified_db.fasta

If path is once determined, it must not be specified again, as long as the same folder shell be used.

Authors

• Juliane Schmachtenberg

project_on_github

License

This project is licensed under the MIT License - see the LICENSE file for details