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Excel report from viral sequencing analysis output

Project description

xlavir

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Excel report from viral sequencing data analysis output from the nf-core/viralrecon or peterk87/nf-virontus Nextflow pipelines.

Features

  • Collect sample results from a nf-core/viralrecon or peterk87/nf-virontus into a Excel report
    • Samtools read mapping stats (flagstat)

    • Mosdepth read mapping coverage information

    • Variant calling information (SnpEff and SnpSift results, VCF file information)

    • Consensus sequences

  • QA/QC of sample analysis results (basic PASS/FAIL based on minimum genome coverage and depth)

  • Nextflow workflow execution information

  • Prepend worksheets from other Excel documents into the report (e.g. cover page/sheet, sample sheet, lab results)

  • Add custom images into worksheets with custom names and descriptions (e.g. phylogenetic tree figure PNG)

Roadmap

  • Bcftools variant calling stats sheet

  • Sample metadata table to merge with certain stats?

  • YAML config to info sheet?

  • coverage chart with controls?

Credits

This package was created with Cookiecutter and the audreyr/cookiecutter-pypackage project template.

History

0.2.0 (2021-03-04)

  • Added header comments with descriptions of field content

  • Added comment to Variant Matrix sheet A1 cell describing what is shown in the matrix

  • Added highlighting of samples failing QC in other sheets

  • Fixed image scaling by determining image size with imageio

  • Added Medaka / Longshot VCF parsing

0.1.1 (2021-02-16)

  • Collect sample results from a nf-core/viralrecon or peterk87/nf-virontus into a Excel report
    • Samtools read mapping stats (flagstat)

    • Mosdepth read mapping coverage information

    • Variant calling information (SnpEff and SnpSift results, VCF file information)

    • Consensus sequences

  • iVar VCF parsing

  • QA/QC of sample analysis results (basic PASS/FAIL based on minimum genome coverage and depth)

  • Nextflow workflow execution information

  • Prepend worksheets from other Excel documents into the report (e.g. cover page/sheet, sample sheet, lab results)

  • Add custom images into worksheets with custom names and descriptions (e.g. phylogenetic tree figure PNG)

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