Genetic Therapy Generator Toolkit

GTGT website

Try out GTGT via the website of the Dutch Center for RNA Therapeutics: https://gtgt.rnatherapy.nl.

Documentation

The documentation is available on http://gtgt.readthedocs.io/.

Caching

To speed up the tool, you can enable caching by setting the GTGT_CACHE environment variable.

Human

gtgt transcript ENST00000241453.12 | jq .

Variant Information

gtgt links "NM_000094.4:c.5299G>C"

Analyze exon skips

Use the analyze entry point to generate all exon skips, and compare them to the wildtype and patient transcripts

# A frameshift deletion in exon 2
$ gtgt analyze ENST00000375549.8:c.100del
{
 "wildtype": 1.0,
 "ENST00000375549.8:c.50_172del": 0.8048779330148124,
 "patient": 0.3513663563397666,
 "ENST00000375549.8:c.167_317del": 0.3513663563397666
}

# An in-frame deletion in exon 2, notice how non of the exon skips have a
# higher score than the patient
$ gtgt analyze ENST00000375549.8:c.100_102del
{
 "wildtype": 1.0,
 "patient": 0.9970458173607387,
 "ENST00000375549.8:c.50_172del": 0.8048779330148124,
 "ENST00000375549.8:c.167_317del": 0.3484121737005053
}


# An in-frame deletion that creates a STOP codon is recognized as
# as highly detrimental
$ gtgt analyze ENST00000452863.10:c.87_89del
{
 "wildtype": 1.0,
 "patient": 0.18847136926335686,
 "ENST00000452863.10:c.659_787del": 0.18847136926335686,
 "ENST00000452863.10:c.782_890del": 0.18847136926335686,
 "ENST00000452863.10:c.885_968del": 0.18847136926335686,
 "ENST00000452863.10:c.963_1019del": 0.18847136926335686,
 "ENST00000452863.10:c.1014_1116del": 0.18847136926335686,
 "ENST00000452863.10:c.1111_1267del": 0.18847136926335686,
 "ENST00000452863.10:c.1262_1357del": 0.18847136926335686,
 "ENST00000452863.10:c.1352_1450del": 0.18847136926335686
}

Disclaimer

Copyright© 2023 LUMC (https://www.lumc.nl)

This program is free software: you can redistribute it and/or modify it under the terms of the GNU Affero General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU Affero General Public License for more details.

By accessing and using the program in any manner (including copying, modifying or redistributing the program), you accept and agree to the applicability of the GNU Affero General Public License. You can find and read this license on GNU Affero General Public License - GNU Project - Free Software Foundation.

In case of questions, you can contact us at DCRT@LUMC.nl.