Skip to main content

stick-like object tracker

Project description


SLOTH (stick-like object tracking high resolution) was created to evaluate the dynamic behavior of biological objects, that have a stick-like shape.
SLOTH provides a tracking module, as well as 2 analysis modules. The analysis module is designed for users interested in the length and shape of the objects.
The tracker was designed for microtubule TIRF movies, but it can easlily be used for any movie like stretched DNA, etc.
You might find it most useful for tasks involving analysing growth speeds and length distributions.
The module contains 3 scripts with a Tkinter based GUI that can be executed processively to track, select and analyse the behavior of objects in a movie /stack of images.


SLOTH contains the following scripts:

* image_analyser: a tracking routine supporting .tif and .nd2 files.

* select_ROI: a viewing module, created to select ROIs from the tracked coordinates

* automatic_analysis: a module to calculate the mean-square displacement from the data.

The tracker supports the input of a drift file, where the drift is specified in a x,y format. This drift will automatically be subtracted while tracking.

B supported formats
At this time sloth supports .tif and .nd2 formats, as created by most microscopy programs.

C installing sloth

D Tested applications

The program is intended for an was tested on dynamic microtubule assays (<> Gell et. al).
The tracking precision is limited by the signal-to-noise ratio, and the identification of object relies on the fact that the objects can be approximated to be very thin.
(There needs to a clear axis.)


B.Kauer has created the module for reading .nd2 files.

B.Kauer for advice, M.Zanic and J.Howard for consultation regarding the analysis module, and B.Schlögl for creative brain-storming.

Project details

Download files

Download the file for your platform. If you're not sure which to choose, learn more about installing packages.

Files for SLOTH, version 0.0.8
Filename, size File type Python version Upload date Hashes
Filename, size SLOTH-0.0.8.tar.gz (45.4 kB) File type Source Python version None Upload date Hashes View

Supported by

AWS AWS Cloud computing Datadog Datadog Monitoring DigiCert DigiCert EV certificate Facebook / Instagram Facebook / Instagram PSF Sponsor Fastly Fastly CDN Google Google Object Storage and Download Analytics Microsoft Microsoft PSF Sponsor Pingdom Pingdom Monitoring Salesforce Salesforce PSF Sponsor Sentry Sentry Error logging StatusPage StatusPage Status page