arcos4py 0.1.4

A python package to detect collective spatio-temporal phenomena.

arcos4py

Arcos4py is a python package to detect collective Spatio-temporal phenomena.

The package is currently in the development phase. Additional features, such as more plotting functionality will come with future updates. This also means that functionality might change in the feature.

• Documentation: https://bgraedel.github.io/arcos4py
• GitHub: https://github.com/bgraedel/arcos4py
• PyPI: https://pypi.org/project/arcos4py/
• Free software: MIT

Features

Automated Recognition of Collective Signalling for python (arcos4py) is a python port of the R package ARCOS (https://github.com/dmattek/ARCOS) to identify collective spatial events in time-series data. The software identifies collective protein activation in 2- and 3D cell cultures and can track events over time. Such collective waves have been recently identified in various biological systems and have been demonstrated to play a crucial role in the maintenance of epithelial homeostasis (Gagliardi et al., 2020, Takeuchi et al., 2020, Aikin et al., 2020), in the acinar morphogenesis (Ender et al., 2020), osteoblast regeneration (De Simone et al., 2021), and the coordination of collective cell migration (Aoki et al., 2017, Hino et al., 2020).

Despite its focus on cell signaling, the framework can also be applied to other spatiotemporally correlated phenomena.

Todo's

• Add additional tests for binarization and de-biasing modules.
• Add example processing to documentation with images of collective events.

Data Format

The time series should be arranged in a long table format where each row defines the object's location, time, and optionally the measurement value.

ARCOS defines an ARCOS object on which several class methods can be used to prepare the data and calculate collective events. Optionally the objects used in the ARCOS class can be used individually by importing them from arcos.tools

Installation

Arcos4py can be installed from PyPI with:

    pip install arcos4py

Napari Plugin

Arcos4py is also available as a Napari Plugin arcos-gui. arcos-gui can simplify parameter finding and visualization.

Credits

Maciej Dobrzynski created the original ARCOS algorithm.

This package was created with Cookiecutter and the waynerv/cookiecutter-pypackage project template.