tile phylogenetic space with subtrees
Project description
azulejo noun INFORMAL a glazed tile, usually blue, found on the inside of churches and palaces in Spain and Portugal.
azulejo
azulejo azulejo tiles phylogenetic space with subtrees normalizes and validates genomic data files prior to further processing or inclusion in a data store such as that of the Legume Federation.
Prerequisites
Python 3.6 or greater is required. This package is tested under Linux and MacOS using Python 3.7.
Installation for Users
Install via pip or (better yet) pipx:
pipx install azulejo
azulejo contains some long commands and many options. To enable command-line completion for azulejo commands, execute the following command if you are using bash as your shell:
eval "$(_AZULEJO_COMPLETE=source_bash azulejo)"
For Developers
If you plan to develop azulejo, you’ll need to install the poetry dependency manager. If you haven’t previously installed poetry, execute the command:
curl -sSL https://raw.githubusercontent.com/python-poetry/poetry/master/get-poetry.py | python
Next, get the master branch from GitHub
git clone https://github.com/legumeinfo/azulejo.git
Change to the azulejo/ directory and install with poetry:
poetry install -v
Run azulejo with poetry:
poetry run azulejo
Usage
Installation puts a single script called azulejo in your path. The usage format is:
azulejo [GLOBALOPTIONS] COMMAND [COMMANDOPTIONS][ARGS]
Global Options
The following options are global in scope and, if used must be placed before COMMAND:
-v, –verbose |
Log debugging info to stderr. |
-q, –quiet |
Suppress logging to stderr. |
–no-logfile |
Suppress logging to file. |
-e, –warnings_as_errors |
Treat warnings as fatal (for testing). |
Commands
A listing of commands is available via azulejo --help. The currently implemented commands are:
add-singletons |
Add singleton clusters to cluster file. |
adjacency-to-graph |
Turn adjacency data into GML graph file. |
analyze-clusters |
Statistics of clustering as function of identity. |
annotate-homology |
Marshal homology and sequence information. |
cluster-in-steps |
Cluster in steps from low to 100% identity. |
clusters-to-histograms |
Compute histograms from cluster file. |
combine-clusters |
Combine synteny and homology clusters, |
compare-clusters |
compare one cluster file with another |
dagchainer-synteny |
Read DAGchainer synteny into homology frames. |
length-std-dist |
Plot length distribution of singletons in clusters |
outlier-length-dist |
Plot length distribution of outliers in clusters. |
prepare-protein-files |
Sanitize and combine protein FASTA files. |
proxy-genes |
Calculate a set of proxy genes from synteny files. |
synteny-anchors |
Calculate synteny anchors. |
usearch-cluster |
Cluster at a global sequence identity threshold. |
Each command has its COMMANDOPTIONS, which may be listed with:
azulejo COMMAND --help
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