FreeTrace 1.6.1.1

FreeTrace — single-molecule tracking with fBm inference

FreeTrace

[!IMPORTANT]
Requirements

• Windows(10/11) / GNU/Linux(Debian/Ubuntu) / MacOS(Sequoia/Tahoe)
  
• C compiler (clang)
  
• Python3.10 ↑
  
• GPU & Cuda12 on GNU/Linux with pre-trained models (recommended)
  

[!NOTE]

• PRE-REQUISITE: pre-installation and compilation, check tutorial.
  
• Check compatibilities of Python and Tensorflow to run FreeTrace with source code.
  
• Without GPU, FreeTrace is slow if it infers under fractional Brownian motion.
  
• Current version is stable with python 3.10 / 3.11 / 3.12
  

Visualised FreeTrace results

  FreeTrace infers individual trajectories from time-series images with reconnection of the detected particles under fBm.

Contact person

[e-mail]

Contributors

If you use this software, please cite it as below.

@article {Park2026.01.08.698486,
	author = {Park, Junwoo and Sokolovska, Nataliya and Cabriel, Cl{\'e}ment and Kobayashi, Asaki and Corsin, Enora and Garcia Fernandez, Fabiola and Izeddin, Ignacio and Min{\'e}-Hattab, Judith},
	title = {Novel estimation of memory in molecular dynamics with extended and comprehensive single-molecule tracking software: FreeTrace},
	elocation-id = {2026.01.08.698486},
	year = {2026},
	doi = {10.64898/2026.01.08.698486},
	publisher = {Cold Spring Harbor Laboratory},
	URL = {https://www.biorxiv.org/content/early/2026/01/10/2026.01.08.698486},
	eprint = {https://www.biorxiv.org/content/early/2026/01/10/2026.01.08.698486.full.pdf},
	journal = {bioRxiv}
}