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Internal lab use tool for 3 end analysis

Project description

jla-demultiplexer: Internal JLA lab tool for processing gene-specific sequencing experiments

Installation

pip install jla-demultiplexer

Usage

fastqbreakdown: Removes duplicate reads and trims barcodes and randommer addition

fastqbreakdown -r1 [read 1 location] -r2 [read 2 location] -r [length of random-mer 10 or 11] -b [barcode, barcode+gene specific sequence from manifest]

Coming soon

  • Handling manifests
  • Aligning
  • Creating tailer-analysis compatible files

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