Internal lab use tool for 3 end analysis
Project description
jla-demultiplexer: Internal JLA lab tool for processing gene-specific sequencing experiments
Installation
pip install jla-demultiplexer
Usage
fastqbreakdown: Removes duplicate reads and trims barcodes and randommer addition
fastqbreakdown -r1 [read 1 location] -r2 [read 2 location] -r [length of random-mer] -b [barcode]
Notes
- Random-mer length supplied should be 10 or 11
- barcode supplied should be barcode column + gene specific column from manifest
Coming soon
- Handling manifests
- Aligning
- Creating tailer-analysis compatible files
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