Functions to extract information from Oxford Nanopore sequencing data and alignments.
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nanoget
This module provides functions to extract useful metrics from Oxford Nanopore sequencing reads and alignments.
FUNCTIONS
Data can be presented in the following formats, using the following functions:
- A sorted bam file
process_bam(bamfile, threads) - A standard fastq file
process_fastq_plain(fastqfile, 'threads') - A fastq file with metadata from MinKNOW or Albacore
process_fastq_rich(fastqfile) - A sequencing_summary file generated by Albacore
process_summary(sequencing_summary.txt, 'readtype')
Fastq files can be compressed using gzip, bzip2 or bgzip. The data is returned as a pandas DataFrame with standardized headernames for convenient extraction. The functions perform logging while being called and extracting data.
INSTALLATION
pip install nanoget
or
conda install -c bioconda nanoget
Copyright: 2016-2020 Wouter De Coster decosterwouter@gmail.com
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