Neurotorchmz 24.11.5

Neurotorch is a tool designed to extract regions of synaptic activity in neurons tagges with iGluSnFR, but is in general capable to find any kind of local brightness increase due to synaptic activity

Please note: There is another project called neurotorch on GitHub/PyPI not related to this project. To avoid mix-up, the package is named neurotorchmz with the mz as a refrence to Mainz where the software was developed.

Neurotorch

Neurotorch is a tool designed to extract regions of synaptic activity in neurons tagges with iGluSnFR, but is in general capable to find any kind of local brightness increase due to synaptic activity. It works with microscopic image series / videos and is able to open an variety of formats (for details see below)

• Fiji/ImageJ: Full connectivity provided. Open files in ImageJ and send them to Neurotorch and vice versa.
• Stimulation extraction: Find the frames where stimulation was applied
• ROI finding: Auto detect regions with high synaptic activity. Export data directly or send the ROIs back to ImageJ
• Image analysis: Analyze each frame of the image and get a visual impression where signal of synapse activity was detected
• API: You can access the core functions of Neurotorch also by importing it as an python module

Installation

You need python to run Neurotorch. Also it is recommended to create a virtual enviorenment to not mess up with your other python packages, for example using miniconda. When inside your virtual enviorenment, simply type

pip install neurotorchmz

Also, you need to install OpenJDK and Apache Maven to run PyImageJ. An easy solution is to use the bundled Build from Microsoft you can find here

To update your installation, type

pip install neurotorchmz --upgrade

Documentation

There is neurotorch_documentation.pdf on the GitHub repository, but you can also access it from inside Neurotorch on the tab 'Welcome to Neurotorch'

About

Neurotorch was developed at the AG Heine (Johannes Gutenberg Universität, Mainz/Germany) and is currently under active development.

Development roadmap

Currently in active development:

• released Integration of plugins: Rather than providing an direct binding to TraceSelector, it will be implemented as a plugin
• New ROI finding algorithm based on local maxima

Ideas for future releases:

• Synapse analysis tab: Same algorithm as in the Synapse ROI finder, but for each signal frame separately

Impressions

Please note: Neurotorch is under continuous development. Therefore the visuals provided here may be outdated in future versions.

First impression of an file opened in Neurotorch. For specific file formats (here nd2), a variety of metadata can be extracted

Use the tab 'Signal' to find the timepoints with stimulation (marked in the plot on the left site with yellow dots). You can also use this tab to view the video frame by frame

Extraction of regions with high synaptic activity. For the choosen image with good enough signal to noise ratio, all settings were determined automatically by the program and nothing more than pressing 'Detect' was necessary to get this screen. The ROIs are marked in the images with red boundaries while the selected ROI displayed also with the mean value over time is marked with yellow boundaries

Release notes

24.11.5 (21.11.2024)

• Bugfix The documentation was not included properly

24.11.4 (21.11.2024)

• Introduction of Plugins: Added the ability to add plugins to neurotorch and introduced TraceSelector as preinstalled plugin
• Cache: Added 'Clear cache' option to denoise menu
• Various bugfixes