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The oligotyping and minimum entropy decomposition (MED) pipeline for the analysis of marker gene amplicons

Project Description

Oligotyping

Oligotyping is a novel supervised computational method to decompose very closely related taxa within microbial communities by utilizing very subtle nucleotide variation among 16S Ribosomal RNA gene amplicon reads. It can reveal previously unobserved ecological patterns, and hidden diversity concealed within environmental datasets.

With oligotyping, depending on the hyper-variable region, similarity threshold between different types can be as low as 0.2%, more than a magnitude lower than de facto 3% similarity threshold that is being used by OTU clustering methods.

For installation and usage instructions, please see the project web page:

Licence

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 2 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program. If not, see <http://www.gnu.org/licenses/>.

Author

I am an assistant research scientist at the [Marine Biological Laboratory](http://mbl.edu), [The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution](http://jbpc.mbl.edu/), in Woods Hole Massachusetts, studying microbial ecology.

http://meren.org

Release History

Release History

This version
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2.1

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2.0

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1.9

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1.8-dev

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1.7

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1.6

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1.4

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1.3

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1.2

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1.1

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1.0

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File Name & Checksum SHA256 Checksum Help Version File Type Upload Date
oligotyping-2.1.tar.gz (2.6 MB) Copy SHA256 Checksum SHA256 Source Jan 23, 2016

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