pwsAI 0.1.1

A GUI for AI segmentation of cell imagery.

Welcome to PWS_AI

This code is incomplete in that the gui is not completely finished. Coming up on the gui is the ability to check the results for the threshold so the segmentation is optimized by the user before going to check it on pwspy analysis software.

As it stands, you will go through 5 main steps.

Step 0) Run analysis on dataset of interest, that means running it on the pws analysis software

Step 1) Select folder with cell data where you just ran your analysis (same way its done on the pwspy software)

Step 2) Access rms images from analysis. This is done automatically when you click the button "Get RMS Images"

Step 3) Use PWS_AI to generate the ROIs automatically and save them as TIF File in Corresponding Folder

Step 4) Conver the PWS_AI output into the pwspy compatible format. Done by clicking "Push ROI TO PWSPY"

Once this is done you can go to the pws analysis software where you can now use the AI generated ROIs. This is a beta version and not ready for release but thank you for testing it out!

• Nico 6/15/23