TITAN

Titan is a comprehensive multi-omics data analysis pipeline. TITAN contains a modular analysis pipeline for assembly, annotation, quantification and genome binning of metagenomics. TITAN transforms raw sequence data into functional and taxonomic data at the microbial population level and provides genome-centric resolution through genome binning. TITAN is user-friendly, easy install through Bioconda maintained as open-source on GitHub.

Initial development phase

Titan is currently in the early phases of development, check back soon for updates.

Installing Titan

Option 1) Anaconda

• Anaconda install from bioconda with all dependencies:

conda create -n titan -c conda-forge -c bioconda titan -y

Option 2) pip

pip install titan_omics

• *Dependencies should be installed manually and specified in the config file or path

Option 3) Manual Install

*Latest code might be unstable

1. Clone github Repo

   git clone https://github.com/raw-lab/titan.git
   

2. Run Setup File

   cd titan
   python3 install_titan.py
   conda activate titan
   

• This creates an anaconda environment called "titan" with all dependencies installed.

Input formats

• From any NextGen sequencing technology (from Illumina, PacBio, Oxford Nanopore)
• type 1 raw reads (.fastq format)
• type 2 nucleotide fasta (.fasta, .fa, .fna, .ffn format), assembled raw reads into contigs
• type 3 protein fasta (.faa format), assembled contigs which genes are converted to amino acid sequence

Citing Titan

CONTACT

The informatics point-of-contact for this project is Dr. Richard Allen White III.
If you have any questions or feedback, please feel free to get in touch by email.
Dr. Richard Allen White III - rwhit101@uncc.edu or raw937@gmail.com.
Jose Figueroa - jlfiguer@uncc.edu
Or open an issue.