FlowCal 1.3.1

Flow Cytometry Calibration Library

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FlowCal

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FlowCal is a library for processing and analyzing flow cytometry data in Python.

It features:

• Extraction of Flow Cytometry Standard (FCS) files into numpy array-like structures

• Traditional and non-standard gating, including automatic density-based two-dimensional gating.

• Traditional transformation functions, such as exponentiation.

• Analysis of calibration beads data, standard curve generation, and transformation to absolute units (Molecules of Equivalent Fluorophore, MEF).

• Plotting, including generation of histograms, density plots and scatter plots.

• A user-fiendly Excel UI to gate, transform, plot, and generate statistics from a list of flow cytometry samples in a simple fashion.

Documentation

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The official documentation can be found in https://flowcal.readthedocs.io. Here you will find installation instructions, tutorials, and more.

Report Bugs

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The official way to report a bug is through the issue tracker on github (https://github.com/taborlab/FlowCal/issues). Try to be as explicit as possible when describing your issue. Ideally, a set of instructions to reproduce the error should be provided, together with the version of all the relevant packages you are using.

Request Features

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Features can also be requested through the issue tracker on github. Try to be as descriptive as possible about the desired feature.