READ
[R]eporter Ion [E]xtractor and [A]nnotation [D]irector
READ is a python-based tool to orchestrate TMTpro-18plex quantification for [single cell] DIA and DDA searches with Chimerys, Spectronaut, and DIA-NN.
READ supports Thermo RAW files via ThermoRawFileParser or mzML files, maps
identified precursors to their corresponding MS1 and MS2 spectra, and then quantifies PSMs and/or proteins. Quantification is done either natively, via OpenMS (recommended), or
the TMT Resolution GUI Tool [1]. Quantification is additionally quality controlled by optionally several filters including precursor co-isolation purity, reporter ion resolution,
minimum reporter signal, and minimum reporter signal-to-noise. Filtering behavior is easily controlled via a human-readable .toml configuration file.
READ can easily be installed via PyPI and run via the command line. We also provide executables with a graphical user interface for Microsoft Windows which can be downloaded here. In-depth information on how to install and run READ can be found on GitHub.
Documentation
Please refer to https://github.com/hgb-bin-proteomics/READ/ for more information.
Contact
In case of questions please contact:
1 maintainer
Metadata
1 maintainer
Release files for IMP-READ 2026.9.18
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Source distribution (sdist)
| File | Size | Uploaded | |
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| imp_read-2026.9.18.tar.gz | 22.9 kB | Details |
Built distribution (wheel)
| File | Interpreter | ABI | Platform | Reset |
|---|---|---|---|---|
| imp_read-2026.9.18-py3-none-any.whl | Python 3 | none | any | Details |
Total release size: 54.3 kB
Release files / imp_read-2026.9.18.tar.gz
| Download URL | imp_read-2026.9.18.tar.gz |
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| Size | 22.9 kB |
| Tags | Source |
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| Tags | Python 3 |
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