MethylPurify_v2
This script is used for predicting subclone purity.
Get Dependent data
Get the version of genome fasta that you mapped your fastqs, we support hg18 and hg19 genome fasta now.
## use built-in script
cd methylpurify/db
bash genome.sh hg18
Get Dependent software
If your system is debian-like system, try:
sudo apt-get install python-numpy
sudo apt-get install samtools
Easy to start
Input: BAM file, this should be mapped with BSMap with -R option * BSMap
Currently, we only support hg19 and hg18 genome index mapped BAM file.
If your fastq mapping is done with hg19 index, use following command:
MethylPurify -f input.bam -b 300 -c 20 -s 50 --species hg19 -g /path/to/hg19.fa
Options
-f: input BAM file
-c: coverage level
-b: genome bin size
-s: sampling times
–species: species genome version
-g: species genome fasta file(correspond to –species)
Metadata
Release files for MethylPurify 2.0-20140310
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| MethylPurify-2.0-20140310.tar.gz | 580.5 kB | Without provenance | Details for MethylPurify-2.0-20140310.tar.gz |
Release files / MethylPurify-2.0-20140310.tar.gz
| Download URL | MethylPurify-2.0-20140310.tar.gz |
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