RNF framework for NGS: simulation of reads, evaluation of mappers, conversion of RNF-compliant data.
Read Naming Format is a generic format for assigning read names with encoded information about original positions. RNFtools is an associated software package which can:
- simulate RNF-compliant reads using a wide class of integrated read simulators (Art, DwgSim, Mason, WgSim, etc.), either from a single genome (i.e., a whole genome sequencing) or multiple genomes (i.e., a metagenomic simulation);
- evaluate mappers using RNF reads;
- convert non-RNF simulated reads to RNF (e.g., from SAM format);
- transform genomic coordinates of RNF reads between different coordinate systems (using chain LiftOver format).