Welcome to the AAanalysis documentation!
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AAanalysis (Amino Acid analysis) is a Python framework for interpretable sequence-based protein prediction. Its foundation are the following algorithms:
CPP: Comparative Physicochemical Profiling, a feature engineering algorithm comparing two sets of protein sequences to identify the set of most distinctive features.
dPULearn: deterministic Positive-Unlabeled (PU) Learning algorithm to enable training on unbalanced and small datasets.
AAclust: k-optimized clustering wrapper framework to select redundancy-reduced sets of numerical scales (e.g., amino acid scales).
In addition, AAanalysis provide functions for loading various protein benchmark datasets, amino acid scales, and their two-level classification (AAontology). We combined CPP with the explainable AI SHAP framework to explain sample level predictions with single-residue resolution.
If you are looking to make publication-ready plots with a view lines of code, see our Plotting Prelude.
You can find the official documentation at Read the Docs.
Install
AAanalysis can be installed from PyPi:
pip install aaanalysisFor extended features, including the explainable AI module:
pip install "aaanalysis[pro]"If you use uv, the equivalent commands are:
uv pip install aaanalysis
uv pip install "aaanalysis[pro]"Contributing
We appreciate bug reports, feature requests, or updates on documentation and code. For details, please refer to Contributing Guidelines. These cover AAanalysis development conventions and the automated quality gates every change must pass. For further questions or suggestions, please email stephanbreimann@gmail.com.
Cheat Sheet
The cheat sheet distills AAanalysis into a three-page summary: the golden workflow, the main classes grouped by capability, the prediction levels (residue / domain / protein), and the Part × Split × Scale feature ontology. Click the image below to download the PDF.
The AAanalysis Ecosystem
AAanalysis is the interpretable middle layer between bioinformatics I/O and the downstream machine learning, explainable AI, and protein-design stack. It consumes upstream representations (sequences, embeddings, structures) and even competitor descriptor sets, and runs them through its interpretable core (Part × Split × Scale · AAontology · CPP). Downstream machine-learning and explainable-AI methods then either consume these features directly or are integrated into AAanalysis through wrappers or native implementations — for example SHAP via ShapModel, or machine-learning models such as random forests via TreeModel — so the resulting features, explanations, and design objectives feed straight into the standard ML / XAI / optimization tools.
Click the diagram to view and download the full map, or open the ecosystem positioning page — a self-contained walkthrough with the map, its introduction, and further background.
Decision Map
Not sure which tool fits your question? The Decision Map routes you from your biological task (residue, domain, or protein level, plus determinant discovery and design) to the right AAanalysis workflow and classes. Click the map to open the full, downloadable version (PNG / PDF / HTML).
Data Flow Map
The Data Flow Map shows how the pieces connect end to end: external inputs (sequences, embeddings, structures, annotations) feed the interpretable CPP core, which turns them into the feature signature df_feat and the feature matrix X that the wrapper classes use to predict, explain, and design. Click the map to open the full, downloadable version.
Citations
If you use AAanalysis in your work, please cite the respective publication as follows:
- AAclust:
Breimann and Frishman (2024a), AAclust: k-optimized clustering for selecting redundancy-reduced sets of amino acid scales, Bioinformatics Advances.
- AAontology:
Breimann et al. (2024b), AAontology: An ontology of amino acid scales for interpretable machine learning, Journal of Molecular Biology.
- CPP:
Breimann and Kamp et al. (2025), Charting γ-secretase substrates by explainable AI, Nature Communications.
- dPULearn:
Breimann and Kamp et al. (2025), Charting γ-secretase substrates by explainable AI, Nature Communications.
1 maintainer
Metadata
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Release files for aaanalysis 1.1.0
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| aaanalysis-1.1.0.tar.gz | 8.9 MB | Details |
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