abi-agent 1.2.0

Agent-Bioinformatics Interface: plugin-based abstraction for AI-driven bioinformatics analysis

ABI — Agent-Bioinformatics Interface

ABI is a Python interface layer for agent-driven bioinformatics workflows. It standardizes analysis plugins behind a common plan -> dry-run -> run -> inspect -> report lifecycle, with provenance, standard TSV tables, multi-LLM tool descriptors (OpenAI, Anthropic Claude, Google Gemini, DeepSeek, 智谱 GLM, Kimi, Qwen, MiniMax), optional MCP transport, Nextflow export/runtime support, DAG/contract static analysis, and a queue-backed HTTP Job Service with force-kill capability.

:cn: 中文版

Installation

pip install abi-agent

# Development install
pip install -e ".[dev]"

# Optional MCP server dependencies
pip install -e ".[dev,mcp]"

Python 3.10-3.13 is supported.

Quick Start

# List installed analysis plugins
abi list-types

# Build a plan without executing tools
abi plan --type metatranscriptomics --config config.yaml --sample-sheet samples.tsv

# Write dry-run provenance and table skeletons
abi dry-run --type metatranscriptomics --config config.yaml --sample-sheet samples.tsv

# Execute only after explicit confirmation
abi run --type metatranscriptomics --config config.yaml --sample-sheet samples.tsv \
  --confirm-execution

# Inspect and rebuild reports
abi inspect --result-dir results/
abi report --result-dir results/ --type metatranscriptomics

# Export agent/runtime interfaces
abi export-nextflow --type metatranscriptomics --output workflow.nf
abi export-openai-tools --type metatranscriptomics --format responses    # legacy compat
abi export-tools --type metatranscriptomics --format openai --provider openai   # OpenAI
abi export-tools --type metatranscriptomics --format openai --provider deepseek # DeepSeek
abi export-tools --type metatranscriptomics --format openai --provider zhipu    # 智谱 GLM
abi export-tools --type metatranscriptomics --format anthropic           # Claude
abi export-tools --type metatranscriptomics --format gemini              # Gemini
abi export-agent-context --type metatranscriptomics --format json
abi doctor-agent --type metatranscriptomics

# Static contract / DAG validation
abi contract-lint --type metagenomic_plasmid
abi contract-lint --type metagenomic_plasmid --strict

# Headless agent dispatch (used by Job Service workers)
abi dispatch --command list-types --arguments '{}'

# Start MCP stdio server for Claude Desktop / Claude Code
abi-mcp

# Install ABI agent skills into Claude Code (~/.claude/skills/abi/)
abi install-skills

# Job Service with optional force-kill subprocess workers
abi job-service --workers 2 --store jobs.json --subprocess-workers

All agent-facing commands support --output-json.

Built-In Analysis Types

Type	Tools	Description
amplicon_16s	8	16S rRNA microbiome: cutadapt → vsearch merge/derep/denoise → SINTAX taxonomy → MAFFT+FastTree phylogeny → diversity (alpha/beta)
rnaseq_expression	6	Bulk RNA-seq: fastp → STAR → featureCounts → build_count_matrix → DESeq2 → clusterProfiler
wgs_bacteria	5	Bacterial isolate WGS: fastp → SPAdes → Prokka → MLST → AMRFinderPlus
metatranscriptomics	3	Metatranscriptomics: fastp → STAR/HISAT2 → featureCounts
metagenomic_plasmid	67	Flagship plasmid analysis: QC → assembly → plasmid detection → annotation → abundance → statistics. 10 conda envs, 84-node DAG.

The autoplasm CLI is preserved for backward compatibility:

autoplasm dry-run --config examples/config_minimal.yaml --profile dry_run

Docker

Pre-built Docker images for all 5 plugins:

# Build a plugin image
docker build -f docker/Dockerfile.amplicon -t abi-amplicon .

# Run a workflow inside the container
docker run --rm -v $PWD:/data abi-amplicon \
  abi plan --type amplicon_16s --outdir /data/results

# Start all services with Docker Compose
docker compose -f docker/docker-compose.yml up -d

Images: abi-amplicon (~1.5 GB), abi-rnaseq (~2.5 GB), abi-wgs (~2.0 GB), abi-metatranscriptomics (~2.0 GB), abi-plasmid (~15 GB). See docker/docker-compose.yml for the full orchestration.

Architecture

Agent Platforms (Claude / ChatGPT / Cursor / CI)
        │
        v
Transport Layer   CLI JSON  │  OpenAI/Anthropic/Gemini Tools  │  MCP  │  HTTP Job API  │  Skills
        │
        v
ABIAgentInterface   plan / dry_run / run / inspect / report / dispatch
        │
        v
ABI Core            schemas  │  provenance  │  permissions  │  diagnostics
                    tables   │  tools       │  executor     │  report
        │
        v
Plugins             amplicon_16s/  rnaseq_expression/  wgs_bacteria/
                    metatranscriptomics/  metagenomic_plasmid/
        │
        v
Runtimes            local  │  Docker  │  Nextflow  │  HPC  │  cloud

Design Principles

Principle	Meaning
Thick Core	Lifecycle, permissions, diagnostics, provenance, standard tables, plugin discovery all live in Core.
Thin Transport	CLI, OpenAI tools, MCP, HTTP only adapt calls — no business logic.
Clean Plugin	Biology logic in plugins, generic mechanisms in Core.
Agent Doesn't Code	Agents call ABI through schemas, descriptors, JSON envelopes, and diagnostic hints.

Agent Transports

ABIAgentInterface is the stable transport-neutral boundary used by:

• CLI JSON through --output-json
• abi dispatch --command <name> --arguments '<json>' for headless subprocess dispatch
• Multi-LLM descriptors from abi export-tools --format openai|anthropic|gemini [--provider ...] covering 7+ providers
• OpenAI-compatible descriptors from abi export-openai-tools (backward compat)
• MCP stdio server via abi-mcp (or python -m abi.mcp.server) — auto-generated from SSOT
• HTTP Job Service via abi job-service and abi job submit/list/status/artifacts/cancel
• Skills via abi install-skills (copies bundled SKILL.md files to ~/.claude/skills/abi/)

Agents can also get operating instructions programmatically:

import abi
print(abi.get_agent_guide())        # compact operating guide for system prompt
print(abi.list_plugins_summary())   # list all installed analysis plugins

Execution tools require explicit confirmation. abi run, abi_run, and Job Service execution submissions return confirmation_required unless confirm_execution=true or --confirm-execution is provided.

Job Service

# Start with in-process workers
abi job-service --host 127.0.0.1 --port 18791 --workers 1 --store jobs.json

# Start with subprocess workers for force-kill support
abi job-service --workers 2 --subprocess-workers

# Client commands
abi job submit --command run --analysis-type metatranscriptomics --confirm-execution
abi job status <JOB_ID>
abi job artifacts <JOB_ID>
abi job cancel <JOB_ID>          # SIGTERM → SIGKILL (3s grace) for subprocess workers

When --subprocess-workers is enabled, each job runs in an isolated abi dispatch process and can be force-killed via SIGTERM on cancel. The job record tracks worker_pid and remote_scheduler_job_id (for HPC/cloud backends).

Development

pip install -e ".[dev]"

ruff check src/ tests/
ruff format --check src/ tests/
mypy src/abi/ --ignore-missing-imports
pytest tests/ -v --tb=short

python -m build
python -m twine check dist/*

Repository-local bioinformatics environments are described under envs/ and resolved from .mamba/envs/<env_name>/bin. Set ABI_MAMBA_ROOT to override the default .mamba root; AUTOPLASM_MAMBA_ROOT remains accepted for compatibility.

More details:

• ABI Spec v0.1
• Development Plan
• API Reference — Sphinx auto-generated from docstrings
• Plugin Development Guide
• RNA-seq Workflow
• Workflow Validation
• HPC Development
• OpenAI/LLM Interface Standard
• Agent Usage Guide
• Job Service Guide
• Release Guide
• Dev Log

Public SDK

Plugin authors should depend on these public modules:

Module	Contents
abi.interfaces	ABIPlugin, ABIDryRunPlugin, ABIInitializablePlugin
abi.schemas	SampleInput, SampleContext, PlanStep, ExecutionPlan (ABI-prefixed aliases available)
abi.tools	ToolRegistry, ToolSkill, GenericCommandSkill, RunResult
abi.provenance	RunLogger, PipelineProgressRecorder, TSV provenance writers
abi.errors	ABIError, ConfigError, SampleSheetError, ToolError, MissingTemplateParamError
abi.contracts	ContractViolationError, validate_output_contract, evaluate_assertions, save_checksums_atomic, run_contract_lint
abi.tool_descriptors	ABI_AGENT_TOOLS, TOOL_ALIASES, export_openai_compatible, export_anthropic, export_gemini, PROVIDER_PROFILES
abi.testing	assert_plugin_contract

Register third-party plugins with:

[project.entry-points."abi.plugins"]
my_analysis = "my_package.plugins:MyPlugin"

License

MIT, see LICENSE.