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The acia library provides utility functionality for analyzing 2D+t time-lapse image sequences in microfluidic live-cell imaging experiments.

Project description

Acia: Automated single-cell image analysis

pipeline coverage

Accio 🪄 - and your single-cell insights appear - Not quite but - acia - and your single-cell insights appear to become much easier 😉

The acia library provides a modular image analysis pipeline utility functionality for analysing 2D+t time-lapse image sequences in microfluidic live-cell imaging experiments. It provides:

  • Abstraction for various image sources (local, OMERO)
  • automated image analysis for instance segmentation and tracking (eight SOTA AI approaches supported out of the box)
  • automated and unit-aware single-object property extraction.
  • extended visualization in videos, charts and interactive charts including segmentation masks and lineage trees

Although the funtionality is developed with microfluidic applications in mind, the library can be used for any objects detected in images.

Note: For examples of its usage please visit our application workflow collection including more than 10 real-world examples: https://github.com/JuBiotech/acia-workflows

Installation

Install acia from pypi:

pip install acia

Developers

  1. Clone this repository
    git clone https://github.com/JuBiotech/acia-core.git
    cd acia-core
    

2.Install acia in development mode

```bash
pip install -e .
```

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