AGR Curation API Client

A unified Python client for Alliance of Genome Resources (AGR) curation APIs.

Features

• Unified Interface: Single client for all AGR curation API endpoints
• Multiple Data Sources: Supports REST API, GraphQL, and direct database access
• Type Safety: Full type hints and Pydantic models for request/response validation
• Entity Search: Partial matching and synonym search for genes, alleles, and other entities
• Ontology Search: Comprehensive search across 45 ontology types (GO, DO, HP, and more)
• Disease Annotations: Query disease associations for genes, alleles, and AGMs across all MODs
• Retry Logic: Automatic retry with exponential backoff for transient failures
• Authentication: Support for API key and Okta token authentication
• Async Support: Built on httpx for both sync and async operations
• Comprehensive Error Handling: Detailed exceptions for different error scenarios

Installation

pip install agr-curation-api-client

For development:

git clone https://github.com/alliance-genome/agr_curation_api_client.git
cd agr_curation_api_client
make install-dev

Bounded allele candidates (0.15.0)

The database client provides search_allele_candidates() and get_allele_candidate_details() without changing the existing get_allele() API.

result = db.search_allele_candidates(
    "H2-Ab1", taxon_curie="NCBITaxon:10090", gene_identifier="MGI:103070",
    attribution_hint="Cyagen", functional_impact_hint="conditional_ready",
    limit=20, discovery_limit=200,
)
details = db.get_allele_candidate_details(["MGI:7584221"])

Candidates include official symbol/full name, synonyms, verified is_allele_of gene associations, functional impacts, mutation types, and ranking reasons. An explicit gene ID or exact gene symbol scopes discovery to that gene's active, public allele associations. Taxon is also a hard scope. The literal query is not rewritten; % and _ are treated literally. Attribution text in full names and structured functional impacts are soft ranking clues, never exclusion rules. Missing annotations are unknown, not evidence against a candidate. Every search candidate remains identity_status="unconfirmed", including exact text matches.

coverage distinguishes the bounded discovered set from the displayed subset. discovery_capped means additional database matches may exist; display_capped means discovered candidates were omitted from this response. database_total is deliberately unset rather than presenting a capped count as an exact total. detail_missing_count reports candidates missing from the subsequent detail query. annotations_capped identifies truncated annotation lists per candidate. Refine the explicit scope/clues, increase the bounded budget when justified, or fetch particular identifiers; this API does not automatically exhaust all pages. Database failures propagate to callers and are not reported as zero matches.

Discovery joins details by the database row ID, not by interchangeable identifier columns. Displayed identifiers prefer a nonempty primary external ID and fall back to a nonempty CURIE. Missing identifiers do not collapse distinct database rows; null functional-impact names are omitted rather than interpreted as impact facts. Explicit gene searches start from exact gene matches and use indexed per-allele annotation lookups, including for short literal text. Unscoped substring searches can still examine many database rows: the discovery budget bounds enrichment and response size, not the number of rows scanned. The statement timeout remains the execution safety bound, and short-query substring behavior is preserved.

Operational environment settings (positive integers):

Authentication

The client supports automatic Okta token generation using the same environment variables as other AGR services:

export OKTA_DOMAIN="your-okta-domain"
export OKTA_API_AUDIENCE="your-api-audience"
export OKTA_CLIENT_ID="your-client-id"
export OKTA_CLIENT_SECRET="your-client-secret"

With these environment variables set, the client will automatically obtain an authentication token when initialized.

Quick Start

Basic Usage

from agr_curation_api import AGRCurationAPIClient, APIConfig

# Option 1: Automatic authentication (requires OKTA env vars)
client = AGRCurationAPIClient()

# Option 2: Manual token configuration
config = APIConfig(
    base_url="https://curation.alliancegenome.org/api",
    okta_token="your-okta-token"  # Optional - will auto-retrieve if not provided
)
client = AGRCurationAPIClient(config)

# Use the client
with client:
    # Get genes from WormBase
    genes = client.get_genes(data_provider="WB", limit=10)
    
    for gene in genes:
        symbol = gene.gene_symbol.get("displayText", "") if gene.gene_symbol else ""
        print(f"{gene.curie}: {symbol}")

Data Source Selection

The client supports multiple data access methods with automatic fallback. By default, it tries database access first (fastest), then GraphQL, then REST API.

# Automatic data source selection (default behavior)
client = AGRCurationAPIClient()
genes = client.get_genes(taxon="NCBITaxon:6239", limit=100)  # Uses database if available

# Force specific data source
client = AGRCurationAPIClient(data_source="db")      # Database only
client = AGRCurationAPIClient(data_source="graphql") # GraphQL only
client = AGRCurationAPIClient(data_source="api")     # REST API only

# Direct database access for advanced queries
gene = client.db.get_gene("WB:WBGene00001234")
allele = client.db.get_allele("WB:WBVar00001234")

Working with Genes

from agr_curation_api import AGRCurationAPIClient, Gene

# Use default configuration
client = AGRCurationAPIClient()

# Get genes from a specific data provider
wb_genes = client.get_genes(data_provider="WB", limit=100)
print(f"Found {len(wb_genes)} WormBase genes")

# Get a specific gene by ID (works with database, GraphQL, or API)
gene = client.get_gene("WB:WBGene00001234")
if gene:
    print(f"Gene: {gene.gene_symbol}")
    print(f"Full name: {gene.gene_full_name}")
    print(f"Species: {gene.taxon}")

# Get all genes (paginated)
all_genes = client.get_genes(limit=5000, page=0)

Gene type filtering (database source)

The curation store's gene table also holds non-gene sequence features — TF_binding_site, sequence_feature, TSS_region, polyA_site and others — which carry a gene symbol annotation but are not genes. Unfiltered, C. elegans returns ~778k objects of which only ~49.5k are genes.

Since 0.14.0 the database source therefore restricts results to genes whose Sequence Ontology gene type is SO:0000704 "gene" or one of its is_a descendants. This applies to client.get_genes(data_source="db") and to DatabaseMethods.get_genes_by_taxon / get_genes_raw.

Upgrading from 0.13.x: this is a behaviour change. Existing calls return fewer rows — the non-gene features above are now excluded, and so are pseudogenes, gene segments and heritable phenotypic markers, which sit outside the "gene" subtree in SO. If you need those, pass so_terms explicitly as shown below. The API and GraphQL sources are unaffected and still return unfiltered results.

from agr_curation_api import GENE_SO_TERM_CURIE, GENE_LIKE_SO_TERM_CURIES

# Default: SO:0000704 "gene" and its is_a descendants
genes = client.get_genes(taxon="NCBITaxon:6239", data_source="db")

# Also include pseudogenes, gene segments and heritable phenotypic markers.
# These three are not is_a descendants of "gene", so they must be named
# explicitly; MGI in particular relies on the latter two.
genes = client.get_genes(
    taxon="NCBITaxon:10090",
    data_source="db",
    so_terms=GENE_LIKE_SO_TERM_CURIES,
)

# Restore pre-0.14 behaviour: no gene-type filtering at all
genes = client.get_genes(taxon="NCBITaxon:6239", data_source="db", so_terms=[])

GENE_LIKE_SO_TERM_CURIES is ("SO:0000704", "SO:0000336", "SO:3000000", "SO:0001500") — gene, pseudogene, gene_segment and heritable_phenotypic_marker. Use the constant rather than hardcoding the CURIEs.

Two exclusions apply regardless of so_terms: obsolete SO gene types never match (TSS_region is flagged obsolete, for instance), and genes with no gene type at all are dropped, because the filter joins through gene.genetype_id. Pass so_terms=[] to disable both along with the filter itself.

DatabaseMethods additionally accepts include_descendants=False for exact-match-only filtering; that parameter is not exposed on client.get_genes.

Working with Species

# Get all species
species_list = client.get_species()

for species in species_list:
    print(f"{species.abbreviation}: {species.display_name}")

# Find a specific species
wb_species = [s for s in species_list if s.abbreviation == "WB"]
if wb_species:
    print(f"WormBase: {wb_species[0].full_name}")

Working with Ontology Terms

# Get GO term root nodes
go_roots = client.get_ontology_root_nodes("goterm")
print(f"Found {len(go_roots)} GO root terms")

# Get children of a specific GO term
children = client.get_ontology_node_children("GO:0008150", "goterm")  # biological_process
for child in children:
    print(f"{child.curie}: {child.name}")

# Get disease ontology terms
disease_roots = client.get_ontology_root_nodes("doterm")

# Get anatomical terms
anatomy_roots = client.get_ontology_root_nodes("anatomicalterm")

Working with Expression Annotations

# Get expression annotations for WormBase
wb_expressions = client.get_expression_annotations(
    data_provider="WB",
    limit=100
)

for expr in wb_expressions:
    if expr.expression_annotation_subject:
        gene_id = expr.expression_annotation_subject.get("primaryExternalId")
        gene_symbol = expr.expression_annotation_subject.get("geneSymbol", {}).get("displayText")
        print(f"Gene: {gene_id} ({gene_symbol})")
        
    if expr.expression_pattern:
        anatomy = expr.expression_pattern.get("whereExpressed", {}).get("anatomicalStructure", {}).get("curie")
        print(f"  Expressed in: {anatomy}")

Working with Disease Annotations

The client provides comprehensive disease annotation queries supporting gene, allele, and AGM (affected genomic model) disease associations.

from agr_curation_api import DatabaseMethods

db = DatabaseMethods()

# Get disease annotations for a specific gene
annotations = db.get_disease_annotations_by_gene("WB:WBGene00004271")  # rab-7

for ann in annotations:
    print(f"Disease: {ann.disease_name} ({ann.disease_curie})")
    print(f"  Relation: {ann.relation}")
    print(f"  Reference: {ann.reference_curie}")

# Include ECO evidence codes
annotations = db.get_disease_annotations_by_gene(
    "WB:WBGene00004271",
    include_evidence_codes=True
)

for ann in annotations:
    if ann.evidence_codes:
        print(f"Evidence: {', '.join(ann.evidence_codes)}")

# Get disease annotations by species and annotation type
# annotation_type: "gene", "allele", or "agm"
worm_gene_diseases = db.get_disease_annotations_by_taxon(
    "NCBITaxon:6239",           # C. elegans
    annotation_type="gene",
    limit=100
)

# Mouse uses allele-level annotations (not gene-level)
mouse_allele_diseases = db.get_disease_annotations_by_taxon(
    "NCBITaxon:10090",          # M. musculus
    annotation_type="allele",
    limit=100
)

# FlyBase uses AGM-level annotations
fly_agm_diseases = db.get_disease_annotations_by_taxon(
    "NCBITaxon:7227",           # D. melanogaster
    annotation_type="agm",
    limit=100
)

# Get annotations for a specific disease
obesity_annotations = db.get_disease_annotations_by_disease(
    "DOID:9970",                # obesity
    annotation_type="gene",     # optional filter
    limit=50
)

# Lightweight dictionary output for performance
raw_results = db.get_disease_annotations_raw(
    "NCBITaxon:6239",
    annotation_type="gene",
    limit=100
)

for r in raw_results:
    print(f"{r['subject_id']}: {r['disease_name']}")

Note on Data Provider Coverage:

Different MODs submit disease annotations at different levels:

Working with Alleles

# Get alleles from a specific data provider
wb_alleles = client.get_alleles(data_provider="WB", limit=50)

for allele in wb_alleles:
    symbol = allele.allele_symbol.get("displayText", "") if allele.allele_symbol else ""
    print(f"{allele.curie}: {symbol}")

# Get a specific allele through the database source. The returned id is the
# durable public.allele / biologicalentity integer ID.
db_client = AGRCurationAPIClient(data_source="db")
allele = db_client.get_allele("WB:WBVar00000001")
if allele:
    print(f"Allele database ID: {allele.id}")

# Preserve zero/one/multiple outcomes when resolving an existing association.
# Callers that require an unambiguous target should proceed only for one result
# whose durable entity IDs agree with entities resolved separately.
associations = db_client.search_allele_gene_associations(
    "WB:WBVar00000001",
    "WB:WBGene00003883",
)
if allele and len(associations) == 1 and associations[0].allele_id == allele.id:
    print(f"Association database ID: {associations[0].association_id}")

Searching Entities

The client provides two search methods with different strengths:

Entity Search (search_entities): Best for user-friendly, autocomplete-style searching

• Database-only (direct SQL queries)
• Partial text matching: "rut" finds "rutabaga", "RUT", "rut-1"
• Automatically searches symbols, full names, and synonyms
• Returns results with relevance scoring (exact > starts-with > contains)
• Use when: searching by partial names, building autocomplete, finding entities by common/historical names
• Supported types: gene, allele, agm, strain, genotype, fish

Generic Search (search_entities): Best for programmatic queries with known field structures

• REST API-based with structured filters
• Exact field matching with precise field paths (e.g., "geneSymbol.displayText")
• Supports complex boolean filter logic
• Returns complete entity objects
• Use when: you know exact field names, need complex filters, require full entity data
• Supports all entity types available in the API

Entity Search

# Search for genes with partial matching
# Example: Find genes containing "rut" in Drosophila
results = client.db.search_entities(
    entity_type='gene',
    search_pattern='rut',
    taxon_curie='NCBITaxon:7227',
    include_synonyms=True,
    limit=10
)

for result in results:
    print(f"{result['entity_curie']}: {result['entity']}")
    print(f"  Match type: {result['match_type']}")  # exact, starts_with, or contains
    print(f"  Relevance: {result['relevance']}")    # 1 (best) to 3 (least)

# Search for alleles without synonyms
allele_results = client.db.search_entities(
    entity_type='allele',
    search_pattern='daf',
    taxon_curie='NCBITaxon:6239',
    include_synonyms=False,
    limit=20
)

# Supported entity types: 'gene', 'allele', 'agm', 'strain', 'genotype', 'fish'
# Results are ordered by relevance (exact matches first, then starts-with, then contains)

Generic Search

# Generic entity search
search_filters = {
    "dataProvider.abbreviation": "WB",
    "geneSymbol.displayText": "daf-16"
}

results = client.search_entities(
    entity_type="gene",
    search_filters=search_filters,
    limit=10
)

print(f"Total results: {results.total_results}")
print(f"Returned: {results.returned_records}")

for gene_data in results.results:
    print(f"Found gene: {gene_data}")

Ontology Term Search

The client provides comprehensive ontology term search with support for 45 different ontology types including GO, DO, HPTerm, and many more.

# Search Gene Ontology terms
go_results = client.db.search_ontology_terms(
    term='apoptosis',
    ontology_type='GOTerm',
    include_synonyms=True,
    limit=10
)

for result in go_results:
    print(f"{result.curie}: {result.name}")
    print(f"  Namespace: {result.namespace}")
    print(f"  Synonyms: {', '.join(result.synonyms[:3])}")

# Search Disease Ontology with exact matching
disease_results = client.db.search_ontology_terms(
    term='diabetes',
    ontology_type='DOTerm',
    exact_match=True,  # Only exact matches
    limit=5
)

# Organism-specific convenience methods
# Search C. elegans anatomy terms
wb_anatomy = client.db.search_anatomy_terms(
    term='pharynx',
    data_provider='WB',  # C. elegans
    limit=5
)

# Search Mouse life stages
mouse_stages = client.db.search_life_stage_terms(
    term='embryonic',
    data_provider='MGI',  # Mouse
    limit=5
)

# Search GO terms by aspect
cellular_components = client.db.search_go_terms(
    term='nucleus',
    go_aspect='cellular_component',  # or 'biological_process', 'molecular_function'
    limit=10
)

# Other convenience methods:
# - search_disease_terms() - Disease Ontology (DO)
# - search_phenotype_terms() - Phenotype ontologies (HP, MP, WBPhenotype)
# - search_chemical_terms() - ChEBI chemical entities
# - search_evidence_terms() - Evidence & Conclusion Ontology (ECO)
# - search_taxon_terms() - NCBI Taxonomy
# - search_sequence_terms() - Sequence Ontology (SO)

Supported ontology types include: APOTerm, ATPTerm, BSPOTerm, BTOTerm, CHEBITerm, CLTerm, CMOTerm, DAOTerm, DOTerm, ECOTerm, EMAPATerm, FBCVTerm, FBDVTerm, GENOTerm, GOTerm, HPTerm, MATerm, MITerm, MMOTerm, MMUSDVTerm, MODTerm, Molecule, MPATHTerm, MPTerm, NCBITaxonTerm, OBITerm, PATOTerm, PWTerm, ROTerm, RSTerm, SOTerm, UBERONTerm, VTTerm, WBBTTerm, WBLSTerm, WBPhenotypeTerm, XBATerm, XBEDTerm, XBSTerm, XCOTerm, XPOTerm, XSMOTerm, ZECOTerm, ZFATerm, ZFSTerm.

Error Handling

from agr_curation_api import (
    AGRAPIError,
    AGRAuthenticationError,
    AGRConnectionError,
    AGRTimeoutError,
    AGRValidationError
)

try:
    reference = client.get_reference("invalid-id")
except AGRAuthenticationError:
    print("Authentication failed - check your credentials")
except AGRValidationError as e:
    print(f"Invalid data: {e}")
except AGRTimeoutError:
    print("Request timed out - try again later")
except AGRConnectionError:
    print("Connection failed - check network")
except AGRAPIError as e:
    print(f"API error: {e}")
    if e.status_code:
        print(f"Status code: {e.status_code}")

Configuration Options

The APIConfig class supports the following options:

• base_url: Base URL for the A-Team Curation API (default: "https://curation.alliancegenome.org/api")
• okta_token: Okta bearer token for authentication (auto-retrieved if not provided)
• timeout: Request timeout in seconds (default: 30)
• max_retries: Maximum retry attempts (default: 3)
• retry_delay: Initial delay between retries in seconds (default: 1)
• verify_ssl: Whether to verify SSL certificates (default: True)
• headers: Additional headers to include in requests

Environment Variables

The client uses the following environment variables for configuration:

• ATEAM_API: Override the default A-Team API URL (default: uses production curation API)
• OKTA_DOMAIN: Your Okta domain (required for automatic authentication)
• OKTA_API_AUDIENCE: Your API audience (required for automatic authentication)
• OKTA_CLIENT_ID: Your Okta client ID (required for automatic authentication)
• OKTA_CLIENT_SECRET: Your Okta client secret (required for automatic authentication)

Development

Running Tests

make test

Code Quality

# Run linting
make lint

# Run type checking
make type-check

# Format code
make format

# Run all checks
make check

Building Documentation

cd docs
make html

Contributing

1. Fork the repository
2. Create a feature branch (git checkout -b feature/amazing-feature)
3. Commit your changes (git commit -m 'feat: add amazing feature')
4. Push to the branch (git push origin feature/amazing-feature)
5. Open a Pull Request

License

This project is licensed under the MIT License - see the LICENSE file for details.

Support

• Issues: GitHub Issues
• Documentation: API Documentation
• Contact: software@alliancegenome.org