cd-analyzer 0.2.0

A tool for analyzing and plotting circular dichroism (CD) temperature-dependent data and calculating melting temperature (Tm).

cd-analysis-tool

Introduction

A Python toolkit for analyzing protein thermal denaturation data from Chirascan™ instruments.

Core Features:

• Parse Binary Files: Reads and interprets proprietary binary data files generated by Chirascan™ software.

• Analyze Thermal Melts: Processes variable-temperature Circular Dichroism (CD) data.

• Calculate Melting Temperature (Tm): Automatically fits experimental data to a sigmoidal model to determine the protein's melting temperature (Tm).

Installation

pip install cd-analyzer

Usage

example 1

from pathlib import Path
from src.cdanalyzer.analysis import parse_dsx,help_read_multifile,plot_dtemp_cd,analyze_and_fit_curves,plot_fitted_curves,extrapolate_and_plot_dg
import pandas as pd
import numpy as np
import matplotlib.pyplot as plt
path = Path(r'D:\Project\others\Qiujun\spectrin\CD\20250328\20250328\ST-WT')
fig,axs = plt.subplots(1,2,figsize=(8,4),dpi=200)
data_arr = help_read_multifile(path)
plot_dtemp_cd(axs[0],data_arr,smooth=3)
res = analyze_and_fit_curves(data_arr)
plot_fitted_curves(res,ax=axs[1],showparams=True)

example 1

from pathlib import Path
from src.cdanalyzer.analysis import parse_dsx,help_read_multifile,plot_dtemp_cd,analyze_and_fit_curves,plot_fitted_curves,extrapolate_and_plot_dg
import pandas as pd
import numpy as np
import matplotlib.pyplot as plt
path = Path(r'D:\Project\others\Qiujun\spectrin\CD\20250328\20250328\ST-WT')
fig,axs = plt.subplots(1,2,figsize=(8,4),dpi=200)
data_arr = help_read_multifile(path)
plot_dtemp_cd(axs[0],data_arr,smooth=3)
res = analyze_and_fit_curves(data_arr)
plot_fitted_curves(res,ax=axs[1],showparams=True)