contig 0.32.0

Agentic bioinformatics analyst: Layer 2 execution/verification/reproducibility engine

Contig

An agentic bioinformatics analyst: it runs the analysis, fixes what breaks, and proves the result.

Contig takes raw sequencing data all the way to a verified, reproducible answer: it selects and runs the right pipeline on your own compute, diagnoses and self-heals failures, verifies the output, and writes a portable record anyone can reproduce.

Quickstart · Usage · How it works · Architecture · Vision · Roadmap · Contributing

_{A real terminal capture (offline demo): a run that OOMs → self-heals → passes QC to a PASS verdict → a signed, verified record.}

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Why Contig

Producing an end-to-end analysis from raw sequencing data needs a rare pairing of domain biology and computational engineering. Roughly 74% of wet-lab scientists have no programming experience (2507.20122), and frontier models still reach only ~17% on real bioinformatics analysis (BixBench, 2503.00096). Turning English into a workflow is the easy, crowded part. Running it, fixing it, and proving it is the unsolved part, and that's Contig.

• 🧬 Goal → pipeline, vetted. Describe your goal in plain language; Contig proposes a curated pipeline + params to approve, and flags problems (no replicates, single-end, missing reference) before a multi-hour run.
• 🩹 Self-healing runs. When a step fails (OOM, a missing index it can build, version mismatch, malformed input), Contig diagnoses it, applies a safe patch, and retries, showing you the repair chain. A risky fix pauses the run for your approval instead of guessing.
• ✅ Verified outputs. Every run ends in an honest verdict (PASS / WARN / FAIL / UNVERIFIED) backed by real checks, never a bare "done", and contig show --explain names the exact checks that drove it. The verdict draws on several independent layers: QC-metric thresholds, structural and integrity checks on the output files, cross-tool concordance (a second independent tool corroborates a germline call set: contig verify --concordance-vcf), and biological-plausibility checks (assay-aware sanity such as the Ti/Tv and het/hom ratios for germline variants). Verification is scoped honestly per assay and never over-claims.
• 📦 Reproducible by default. Each run pins inputs (checksums), pipeline revision, params, and tool versions into a portable record. Reproduce any past run with one command or one click. Your reads never leave your machine; only hashes are recorded.
• 📈 Gets better as it runs. Every recovered failure becomes a labeled data point in a versioned failure corpus that compounds, independent of any single model, and a tracked accuracy trend shows the detector improving.
• 🖥 Watch and steer, or run headless. A local dashboard streams live task progress and the self-heal feed, and lets you approve patches, cancel, resume, and reproduce runs; the same controls are CLI commands for scripts and servers.
• ☁️ Same run, laptop or cloud. One command lands unchanged on Docker locally or AWS Batch in your own account, via Nextflow's native executors.

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🎥 A closer look

Four things no incumbent does, each a real command on your own compute.

Self-heal a broken step — diagnose the failure, patch it, retry, unattended.

A verdict you can trust — independent checks, honestly scoped per assay.

Watch and steer, or run headless — live progress and an approval gate for risky fixes.

Gets better as it runs — every failure compounds into a versioned corpus.

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📦 Installation

Pick whichever fits your machine (each release ships all four):

# Python (any OS with Python 3.12+)
pipx install contig            # or: uvx contig --help

# Standalone binary (no Python needed): download from the latest release
#   https://github.com/haqaliz/contig/releases/latest
#   contig-linux-x86_64 | contig-macos-arm64 | contig-macos-x86_64 | contig-windows-x86_64.exe
chmod +x contig-macos-arm64 && ./contig-macos-arm64 --help

# Container (Docker Hub or GitHub Container Registry, same image)
docker run --rm haqaliz/contig:latest --help
docker run --rm ghcr.io/haqaliz/contig:latest --help

# Homebrew (macOS / Linux)
brew install haqaliz/contig/contig

From source, with uv:

git clone https://github.com/haqaliz/contig.git && cd contig
uv sync && uv run contig --help

Running a real pipeline also needs Nextflow, a Java runtime (JAVA_HOME), and a running container runtime. The CLI and its self-contained commands (plan, show, verify, benchmark, eval-detector, ...) work without them. Full prerequisites are in docs/USAGE.md, and the release process is in RELEASING.md.

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🚀 Quickstart

Try it in 30 seconds, no data of your own (uses nf-core/rnaseq's bundled test profile):

contig run --run-id smoke      # run → self-heal → verify → reproduce
contig show smoke              # verdict + provenance + repair chain

Run on your data: plan first, then run against a reference:

contig plan --goal "find differentially expressed genes" \
  --input samplesheet.csv --genome GRCh38      # propose a pipeline to approve

contig run  --run-id my-analysis \
  --input samplesheet.csv --genome GRCh38      # or: --fasta ref.fa --gtf genes.gtf

Running from a source checkout instead of an install? Prefix each command with uv run (e.g. uv run contig run --run-id smoke).

Prefer a screen? The local dashboard launches runs and shows live progress, the self-heal feed, verdict explanations, and the detector trend:

cd dashboard && npm install && npm run dev      # http://localhost:3000 (localhost-only, no auth)

The full sample-sheet format, cloud backends, the reproducible bundle, the live controls, and the failure-corpus workflow are all in docs/USAGE.md.

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🔍 How it works

Contig is built around Layer 2 (the run-and-verify engine) and consumes Layer-1 workflow generation as a replaceable commodity.

  goal + data ──▶ plan ──▶ run ──▶ ⚠ failure? ──▶ self-heal ──▶ verify ──▶ verdict + record
                  (vet)   (your    diagnose →     (patch &      (QC        (PASS/WARN/
                          compute)  classify       retry)        checks)     FAIL/UNVERIFIED)

Verdict	Meaning
PASS	Ran to completion and every QC check passed
WARN	Completed, but a QC check is borderline; look before you trust it
FAIL	A task or QC check failed; do not trust the output
UNVERIFIED	Completed, but nothing checked it, so we won't claim it's correct

Supported analyses

Goal	Pipeline	QC checks	Maturity
RNA-seq differential expression	nf-core/rnaseq	alignment/assignment rate, library-size skew, replicate checks	validated end-to-end
Single-cell RNA-seq	nf-core/scrnaseq	estimated cells, median genes per cell, reads in cells, mito fraction	wired · QC pack
Germline variant calling (research)	nf-core/sarek	Ti/Tv & het/hom ratios, coverage	wired · QC pack
Somatic variant calling (tumor–normal, research)	nf-core/sarek	Strelka2-vs-Mutect2 concordance, VAF distribution, panel-of-normals	wired · QC pack
Methylation (bisulfite)	nf-core/methylseq	bisulfite conversion rate, mapping efficiency, duplication	wired · QC pack
16S amplicon (microbiome)	nf-core/ampliseq	DADA2 read retention, ASV count, sample read depth	wired · QC pack
Shotgun metagenomics	nf-core/mag	assembly N50, bin completeness, contamination	wired · QC pack

contig plan routes to the right one and declines goals it has no curated pipeline for rather than inventing a workflow. The same run → self-heal → verify → reproduce engine serves every assay above. Maturity is honest: nf-core/rnaseq is exercised end-to-end (including the self-heal loop) in CI; the others ship a curated pipeline + assay-aware QC pack and are being hardened toward the same end-to-end bar (see docs/ROADMAP.md).

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📚 Documentation

Document	What's in it
docs/USAGE.md	Full CLI reference, your-own-data walkthrough, the dashboard, live controls (watch, approve, cancel, resume), cloud backends, reproduce/share, failure corpus
CONTRIBUTING.md	Dev setup, package management (uv), tests, project layout, how to contribute
VISION.md	The narrative thesis, the moat, why now, non-goals
docs/RESEARCH_FINDINGS.md	The validated evidence base behind the bet
docs/ROADMAP.md	Phased plan from validation to MVP and beyond
docs/product/PRODUCT_SPEC.md	Product surface, flows, and behavior
docs/technical/ARCHITECTURE.md	The agentic execution/verification system design
docs/business/	Market analysis, business model, go-to-market

Some documents are placeholders being filled in during the validation phase.

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🛠 Status & roadmap

MVP engine built; in early access. The Layer-2 core (run → capture → self-heal → verify → reproduce) works end-to-end on nf-core/rnaseq, built test-first, as a CLI and a local dashboard (launch, live progress, the self-heal approval gate, verdict explainability, one-click reproduce, and the detector-accuracy trend). Not yet built: an LLM-backed planner (the goal→pipeline matcher is deterministic and replaceable today), more assays, hosted multi-user access (the dashboard is localhost-only today), and live-tested slurm/gcp_batch/k8s backends (the mapping layer is in place; local and aws_batch are wired). See docs/ROADMAP.md.

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🤝 Contributing

Contributions are welcome: code, curated pipelines, QC checks, and especially failure cases for the corpus. Start with CONTRIBUTING.md, then open an issue or a pull request.

📄 License

Apache License 2.0 — permissive, with an explicit patent and trademark grant. Use, modify, and redistribute Contig, including commercially, under its terms. Contributions are accepted under the same license (see CONTRIBUTING.md). Contig orchestrates third-party pipelines (nf-core, Nextflow) that carry their own licenses; see NOTICE.

_{Built test-first. The moat is execution, verification, and reproducibility, the part that gets better as models improve.}