crispr-toolkit 0.1.0

A pure-Python utility library for CRISPR and prime editing sequence design and validation.

crispr-toolkit

A small, pure-Python utility library for CRISPR and prime-editing sequence design, validation, and basic scoring. This package is intended as a clean, well-tested FOSS backend for browser tools and scripting workflows.

This project is fully independent from Helix Studio internals and is provided as a standalone, open-source utility library.

Installation

python -m pip install crispr-toolkit

Quickstart

from crispr_toolkit import (
    normalize_seq,
    find_pam_sites,
    validate_guide,
    design_pegRNA,
    basic_on_target_score,
)

seq = normalize_seq("acgtacgtGGGcttacggt")
sites = find_pam_sites(seq, "NGG")
result = validate_guide(seq[2:24])

print(sites)
print(result.is_valid, result.gc_content)

# Example: simple prime editing scaffold
components = design_pegRNA(
    ref_seq=seq,
    edit_pos=5,
    edit_type="substitution",
    edit_seq="A",
)
print(components.pbs, components.rtt)

# Example: basic rule-based on-target score
score = basic_on_target_score(seq[2:22])
print(score)

Roadmap

Planned future features include:

• More PAM presets and convenience helpers.
• Simple off-target enumeration utilities.
• Additional rule-based prime editing recipes and presets.

License

This project is released under the MIT License. See LICENSE for details.