crispr-toolkit
A small, pure-Python utility library for CRISPR and prime-editing sequence design, validation, and basic scoring. This package is intended as a clean, well-tested FOSS backend for browser tools and scripting workflows.
This project is fully independent from Helix Studio internals and is provided as a standalone, open-source utility library.
Installation
python -m pip install crispr-toolkit
Quickstart
from crispr_toolkit import (
normalize_seq,
find_pam_sites,
validate_guide,
design_pegRNA,
basic_on_target_score,
)
seq = normalize_seq("acgtacgtGGGcttacggt")
sites = find_pam_sites(seq, "NGG")
result = validate_guide(seq[2:24])
print(sites)
print(result.is_valid, result.gc_content)
# Example: simple prime editing scaffold
components = design_pegRNA(
ref_seq=seq,
edit_pos=5,
edit_type="substitution",
edit_seq="A",
)
print(components.pbs, components.rtt)
# Example: basic rule-based on-target score
score = basic_on_target_score(seq[2:22])
print(score)
Roadmap
Planned future features include:
- More PAM presets and convenience helpers.
- Simple off-target enumeration utilities.
- Additional rule-based prime editing recipes and presets.
License
This project is released under the MIT License. See LICENSE for details.
1 maintainer
Metadata
1 maintainer
Release files for crispr-toolkit 0.1.0
For a detailed explanation of source distributions (sdists) and built distributions (wheels), please see the package formats documentation.
Source distribution (sdist)
| File | Size | Uploaded | Provenance | Actions |
|---|---|---|---|---|
| crispr_toolkit-0.1.0.tar.gz | 9.0 kB | With provenance | Details for crispr_toolkit-0.1.0.tar.gz |
Built distribution (wheel)
| File | Interpreter | ABI | Platform | Actions Reset |
|---|---|---|---|---|
| crispr_toolkit-0.1.0-py3-none-any.whl | Python 3 | none | any | Details for crispr_toolkit-0.1.0-py3-none-any.whl |
Total release size: 18.2 kB
Release files / crispr_toolkit-0.1.0.tar.gz
| Download URL | crispr_toolkit-0.1.0.tar.gz |
|---|---|
| Size | 9.0 kB |
| Tags | Source |
|
SHA-256 checksum How to use checksums |
bea2b0c780352a0582effd9d3fefaf6ae8f7c693af32c847faa5714767832fe4
|
|
BLAKE2b-256 checksum How to use checksums |
36a5c8b42371d8837350fd5f99df6124fb58b92487b6ddbe01b486fc423c4537
|
| Upload date | |
|
Uploaded using Trusted Publishing? What is trusted publishing? |
Yes |
| Uploaded via |
twine/6.1.0 CPython/3.13.7
|
Provenance
Provenance describes where a file came from. On PyPI, provenance is shared via attestations, which provide a verifiable record of the build or publishing details. View details, limitations and caveats.
PyPI Publish Attestation
PyPI verified that this artifact, at this checksum, originated from the publisher listed below.
Signed by GitHub Actions, verified by PyPI on Nov 29, 2025.
Transparency logRelease files / crispr_toolkit-0.1.0-py3-none-any.whl
| Download URL | crispr_toolkit-0.1.0-py3-none-any.whl |
|---|---|
| Size | 9.2 kB |
| Tags | Python 3 |
|
SHA-256 checksum How to use checksums |
2ee5fbd360379421cc0da2a0eb4215d227f02498ec922508a9b2b5ad51ea7bd2
|
|
BLAKE2b-256 checksum How to use checksums |
d83c69b84174330ca573a9654cbe08118747f82979c88bdd8270fcb06ba5900f
|
| Upload date | |
|
Uploaded using Trusted Publishing? What is trusted publishing? |
Yes |
| Uploaded via |
twine/6.1.0 CPython/3.13.7
|
Provenance
Provenance describes where a file came from. On PyPI, provenance is shared via attestations, which provide a verifiable record of the build or publishing details. View details, limitations and caveats.
PyPI Publish Attestation
PyPI verified that this artifact, at this checksum, originated from the publisher listed below.
Signed by GitHub Actions, verified by PyPI on Nov 29, 2025.
Transparency logSecurity for crispr-toolkit 0.1.0
Provenance
Provenance describes where a file came from. On PyPI, provenance is shared via attestations, which provide a verifiable record of the build or publishing details. View details, limitations and caveats.
Full provenance
All 2 files in this release have been uploaded with provenance metadata.
Attestation for crispr_toolkit-0.1.0.tar.gz
PyPI Publish Attestation
PyPI verified that this artifact, at this checksum, originated from the publisher listed below.
Signed by GitHub Actions, verified by PyPI on Nov 29, 2025.
Transparency logA note on package security
PyPI is a repository for distributing packages, not a service for auditing them. While third-party security researchers and our staff do their best to detect and remove malicious packages, PyPI cannot guarantee the quality, security or suitability of any given package.
To learn more about protecting yourself from malicious actors, visit our package security documentation.
