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Documentation: https://culebront.readthedocs.io/en/latest/

Which assembler gives the best genome for my organism? CulebrONT runs several long-read assemblers side by side on the same reads (Canu, Flye, hifiasm, Miniasm, NextDenovo, Raven, Shasta, SmartDenovo), polishes and corrects their assemblies (Racon, Medaka, Pilon), evaluates them (BUSCO, QUAST, Merqury, BlobToolKit, Assemblytics, GCI, LAI, GenomeScope2…) and compares them in one offline HTML report. It is a Snakemake workflow that runs on a single machine or on a Slurm cluster, with an apptainer image or the cluster modules.

python3 -m pip install culebrONT
culebrONT install --mode local                       # or: culebrONT install (Slurm)
culebrONT test --dataset ecoli -d ~/culebrONT_test
culebrONT run -c ~/culebrONT_test/data_test_config_ecoli.yaml
# choose the assemblies to evaluate in OUTPUT/assembly_selection.tsv, then:
culebrONT quality -c ~/culebrONT_test/data_test_config_ecoli.yaml

Citation

Orjuela J, Comte A, Ravel S, Charriat F, Vi T, Sabot F, Cunnac S. CulebrONT: a streamlined long reads multi-assembler pipeline for prokaryotic and eukaryotic genomes. https://doi.org/10.1101/2021.07.19.452922

Authors

Maintainers: Julie Orjuela (IRD), Aurore Comte (IRD), Sébastien Ravel (CIRAD).

Authors: Julie Orjuela (IRD), Aurore Comte (IRD), Sébastien Ravel (CIRAD), Florian Charriat (INRAE), Tram Vi (IRD, AGI), François Sabot (IRD) and Sébastien Cunnac (IRD).

Thanks

Thanks to Ndomassi Tando (i-Trop IRD) for the administration support.

The authors acknowledge the IRD i-Trop HPC (South Green Platform) at IRD Montpellier for providing HPC resources that have contributed to this work.

Thanks to Yann Delorme for this beautiful logo.

License

Licensed under CeCill-C and GPLv3.

Intellectual property belongs to IRD, CIRAD and authors.

Metadata

Release files for culebrONT 5.0.1

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Source distribution for culebrONT 5.0.1
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