fsqc 2.0.1

Quality control scripts for FastSurfer and FreeSurfer structural MRI data

fsqc toolbox

Description

This package provides quality assurance / quality control scripts for FastSurfer- or FreeSurfer-processed structural MRI data.

It is a revision, extension, and translation to the Python language of the Freesurfer QA Tools. It has been augmented by additional functions from the MRIQC toolbox, and with code derived from the LaPy and BrainPrint toolboxes.

The core functionality of this toolbox is to compute the following features:

variable	description
subject	subject ID
wm_snr_orig	signal-to-noise ratio for white matter in orig.mgz
gm_snr_orig	signal-to-noise ratio for gray matter in orig.mgz
wm_snr_norm	signal-to-noise ratio for white matter in norm.mgz
gm_snr_norm	signal-to-noise ratio for gray matter in norm.mgz
cc_size	relative size of the corpus callosum
lh_holes	number of holes in the left hemisphere
rh_holes	number of holes in the right hemisphere
lh_defects	number of defects in the left hemisphere
rh_defects	number of defects in the right hemisphere
topo_lh	topological fixing time for the left hemisphere
topo_rh	topological fixing time for the right hemisphere
con_lh_snr	wm/gm contrast signal-to-noise ratio in the left hemisphere
con_rh_snr	wm/gm contrast signal-to-noise ratio in the right hemisphere
rot_tal_x	rotation component of the Talairach transform around the x axis
rot_tal_y	rotation component of the Talairach transform around the y axis
rot_tal_z	rotation component of the Talairach transform around the z axis

The program will use an existing output directory (or try to create it) and write a csv table into that location. The csv table will contain the above metrics plus a subject identifier.

The program can also be run on images that were processed with FastSurfer (v1.1 or later) instead of FreeSurfer. In that case, simply add a --fastsurfer switch to your shell command. Note that FastSurfer's full processing stream must have been run, including surface reconstruction (i.e. brain segmentation alone is not sufficient).

In addition to the core functionality of the toolbox there are several optional modules that can be run according to need:

• screenshots module

This module allows for the automated generation of cross-sections of the brain that are overlaid with the anatomical segmentations (asegs) and the white and pial surfaces. These images will be saved to the 'screenshots' subdirectory that will be created within the output directory. These images can be used for quickly glimpsing through the processing results. Note that no display manager is required for this module, i.e. it can be run on a remote server, for example.

• surfaces module

This module allows for the automated generation of surface renderings of the left and right pial and inflated surfaces, overlaid with the aparc annotation. These images will be saved to the 'surfaces' subdirectory that will be created within the output directory. These images can be used for quickly glimpsing through the processing results. Note that no display manager is required for this module, i.e. it can be run on a remote server, for example.

• skullstrip module

This module allows for the automated generation cross-sections of the brain that are overlaid with the colored and semi-transparent brainmask. This allows to check the quality of the skullstripping in FreeSurfer. The resulting images will be saved to the 'skullstrip' subdirectory that will be created within the output directory.

• fornix module

This is a module to assess potential issues with the segmentation of the corpus callosum, which may incorrectly include parts of the fornix. To assess segmentation quality, a screenshot of the contours of the corpus callosum segmentation overlaid on the norm.mgz will be saved as 'cc.png' for each subject within the 'fornix' subdirectory of the output directory.

• modules for the amygdala, hippocampus, and hypothalamus

These modules evaluate potential missegmentations of the amygdala, hippocampus, and hypothalamus. To assess segmentation quality, screenshots will be created These modules require prior processing of the MR images with FreeSurfer's dedicated toolboxes for the segmentation of the amygdala and hippocampus, and the hypothalamus, respectively.

• shape module

The shape module will run a shapeDNA / brainprint analysis to compute distances of shape descriptors between lateralized brain structures. This can be used to identify discrepancies and irregularities between pairs of corresponding structures. The results will be included in the main csv table, and the output directory will also contain a 'brainprint' subdirectory.

• outlier module

This is a module to detect extreme values among the subcortical ('aseg') segmentations as well as the cortical parcellations. If present, hypothalamic and hippocampal subsegmentations will also be included.

The outlier detection is based on comparisons with the distributions of the sample as well as normative values taken from the literature (see References).

For comparisons with the sample distributions, extreme values are defined in two ways: nonparametrically, i.e. values that are 1.5 times the interquartile range below or above the 25th or 75th percentile of the sample, respectively, and parametrically, i.e. values that are more than 2 standard deviations above or below the sample mean. Note that a minimum of 10 supplied subjects is required for running these analyses, otherwise NaNs will be returned.

For comparisons with the normative values, lower and upper bounds are computed from the 95% prediction intervals of the regression models given in Potvin et al., 1996, and values exceeding these bounds will be flagged. As an alternative, users may specify their own normative values by using the '--outlier-table' argument. This requires a custom csv table with headers label, upper, and lower, where label indicates a column of anatomical names. It can be a subset and the order is arbitrary, but naming must exactly match the nomenclature of the 'aseg.stats' and/or '[lr]h.aparc.stats' file. If cortical parcellations are included in the outlier table for a comparison with aparc.stats values, the labels must have a 'lh.' or 'rh.' prefix. file. upper and lower are user-specified upper and lower bounds.

The main csv table will be appended with the following summary variables, and more detailed output about will be saved as csv tables in the 'outliers' subdirectory of the main output directory.

variable	description
n_outliers_sample_nonpar	number of structures that are 1.5 times the IQR above/below the 75th/25th percentile
n_outliers_sample_param	number of structures that are 2 SD above/below the mean
n_outliers_norms	number of structures exceeding the upper and lower bounds of the normative values

---

Usage

As a command line tool

run_fsqc --subjects_dir <directory> --output_dir <directory>
    [--subjects SubjectID [SubjectID ...]]
    [--subjects-file <file>] [--screenshots]
    [--screenshots-html] [--surfaces] [--surfaces-html]
    [--skullstrip] [--skullstrip-html]
    [--fornix] [--fornix-html] [--hippocampus]
    [--hippocampus-html] [--hippocampus-label ... ]
    [--hypothalamus] [--hypothalamus-html] [--shape]
    [--outlier] [--fastsurfer] [-h] [--more-help]
    [...]


required arguments:
  --subjects_dir <directory>
                         subjects directory with a set of Freesurfer- or
                         Fastsurfer-processed individual datasets.
  --output_dir <directory>
                         output directory

optional arguments:
  --subjects SubjectID [SubjectID ...]
                         list of subject IDs
  --subjects-file <file> filename of a file with subject IDs (one per line)
  --screenshots          create screenshots of individual brains
  --screenshots-html     create screenshots of individual brains incl.
                         html summary page
  --surfaces             create screenshots of individual brain surfaces
  --surfaces-html        create screenshots of individual brain surfaces
                         and html summary page
  --skullstrip           create screenshots of individual brainmasks
  --skullstrip-html      create screenshots of individual brainmasks and
                         html summary page
  --fornix               check fornix segmentation
  --fornix-html          check fornix segmentation and create html summary
                         page of fornix evaluation
  --hypothalamus         check hypothalamic segmentation
  --hypothalamus-html    check hypothalamic segmentation and create html
                         summary page
  --hippocampus          check segmentation of hippocampus and amygdala
  --hippocampus-html     check segmentation of hippocampus and amygdala
                         and create html summary page
  --hippocampus-label    specify label for hippocampus segmentation files
                         (default: T1.v21). The full filename is then
                         [lr]h.hippoAmygLabels-<LABEL>.FSvoxelSpace.mgz
  --shape                run shape analysis
  --outlier              run outlier detection
  --outlier-table        specify normative values (only in conjunction with
                         --outlier)
  --fastsurfer           use FastSurfer instead of FreeSurfer output
  --exit-on-error        terminate the program when encountering an error;
                         otherwise, try to continue with the next module or
                         case

getting help:
  -h, --help            display this help message and exit
  --more-help           display extensive help message and exit

expert options:
  --screenshots_base <image>
                        filename of an image that should be used instead of
                        norm.mgz as the base image for the screenshots. Can be
                        an individual file (which would not be appropriate for
                        multi-subject analysis) or can be a file without
                        pathname and with the same filename across subjects
                        within the 'mri' subdirectory of an individual
                        FreeSurfer results directory (which would be appropriate
                        for multi-subject analysis).
  --screenshots_overlay <image>
                        path to an image that should be used instead of aseg.mgz
                        as the overlay image for the screenshots; can also be
                        none. Can be an individual file (which would not be
                        appropriate for multi-subject analysis) or can be a file
                        without pathname and with the same filename across
                        subjects within the 'mri' subdirectory of an individual
                        FreeSurfer results directory (which would be appropriate
                        for multi-subject analysis).
  --screenshots_surf <surf> [<surf> ...]
                        one or more surface files that should be used instead
                        of [lr]h.white and [lr]h.pial; can also be none. Can be
                        one or more individual file(s) (which would not be
                        appropriate for multi-subject analysis) or can be a
                        (list of) file(s) without pathname and with the same
                        filename across subjects within the 'surf' subdirectory
                        of an individual FreeSurfer results directory (which
                        would be appropriate for multi-subject analysis).
  --screenshots_views <view> [<view> ...]
                        one or more views to use for the screenshots in the form
                        of x=<numeric> y=<numeric> and/or z=<numeric>. Order
                        does not matter. Default views are x=-10 x=10 y=0 z=0.
  --screenshots_layout <rows> <columns>
                        layout matrix for screenshot images.

Examples:

• Run the QC pipeline for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory

• Run the QC pipeline for two specific subjects that need to be present in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --subjects mySubjectID1 mySubjectID2

• Run the QC pipeline for all subjects found in /my/subjects/directory after full FastSurfer processing:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --fastsurfer

• Run the QC pipeline plus the screenshots module for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --screenshots

• Run the QC pipeline plus the fornix pipeline for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --fornix

• Run the QC pipeline plus the shape analysis pipeline for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --shape

• Run the QC pipeline plus the outlier detection module for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --outlier

• Run the QC pipeline plus the outlier detection module with a user-specific table of normative values for all subjects found in /my/subjects/directory:

run_fsqc --subjects_dir /my/subjects/directory --output_dir /my/output/directory --outlier --outlier-table /my/table/with/normative/values.csv

• Note that the --screenshots, --fornix, --shape, and --outlier (and other) arguments can also be used in conjunction.

As a Python package

As an alternative to their command-line usage, the fsqc scripts can also be run within a pure Python environment, i.e. installed and imported as a Python package.

Use import fsqc (or sth. equivalent) to import the package within a Python environment, and use the run_fsqc function from the fsqc module to run an analysis.

In its most basic form:

import fsqc
fsqc.run_fsqc(subjects_dir='/my/subjects/dir', output_dir='/my/output/dir')

Specify subjects as a list:

import fsqc
fsqc.run_fsqc(subjects_dir='/my/subjects/dir', output_dir='/my/output/dir', subjects=['subject1', 'subject2', 'subject3'])

And as a more elaborate example:

import fsqc
fsqc.run_fsqc(subjects_dir='/my/subjects/dir', output_dir='/my/output/dir', subject_file='/my/subjects/file.txt', screenshots_html=True, surfaces_html=True, skullstrip_html=True, fornix_html=True, hypothalamus_html=True, hippocampus_html=True, hippocampus_label="T1.v21", shape=True, outlier=True)

Call help(fsqc.run_fsqc) for further usage info and additional options.

---

Citations

• Esteban O, Birman D, Schaer M, Koyejo OO, Poldrack RA, Gorgolewski KJ; 2017; MRIQC: Advancing the Automatic Prediction of Image Quality in MRI from Unseen Sites; PLOS ONE 12(9):e0184661; doi:10.1371/journal.pone.0184661.

• Wachinger C, Golland P, Kremen W, Fischl B, Reuter M; 2015; BrainPrint: a Discriminative Characterization of Brain Morphology; Neuroimage: 109, 232-248; doi:10.1016/j.neuroimage.2015.01.032.

• Reuter M, Wolter FE, Shenton M, Niethammer M; 2009; Laplace-Beltrami Eigenvalues and Topological Features of Eigenfunctions for Statistical Shape Analysis; Computer-Aided Design: 41, 739-755; doi:10.1016/j.cad.2009.02.007.

• Potvin O, Mouiha A, Dieumegarde L, Duchesne S, & Alzheimer's Disease Neuroimaging Initiative; 2016; Normative data for subcortical regional volumes over the lifetime of the adult human brain; Neuroimage: 137, 9-20; doi.org/10.1016/j.neuroimage.2016.05.016