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Hemiplasy Inference Simulation Tool. For characterising hemiplasy given traits mapped onto a species tree

Project description

HeIST

Hemiplasy Inference Simulation Tool

Authors:

Matt Gibson (gibsomat@indiana.edu)
Mark Hibbins (mhibbins@indiana.edu)

Dependencies:

Installation

git clone https://github.com/mhibbins/heist
cd heist
python setup.py install

Usage

 _   _      ___ ____ _____
| | | | ___|_ _/ ___|_   _|
| |_| |/ _ \| |\___ \ | |
|  _  |  __/| | ___) || |
|_| |_|\___|___|____/ |_|
Hemiplasy Inference Simulation Tool
Version 0.3.0

Written by Mark Hibbins & Matt Gibson
Indiana University

usage: heist [-h] [-v] [-n] [-t] [-p] [-g] [-s] [-o] input

Tool for characterising hemiplasy given traits mapped onto a species tree

positional arguments:
  input                 Input NEXUS file

optional arguments:
  -h, --help            show this help message and exit
  -v, --verbose         Enable debugging messages to be displayed
  -n , --replicates     Number of replicates per batch
  -t , --threads        Number of threads for simulations
  -p , --mspath         Path to ms (if not in user path)
  -g , --seqgenpath     Path to seq-gen (if not in user path)
  -s , --mutationrate   Seq-gen mutation rate (default 0.05)
  -o , --outputdir      Output directory/prefix

Input file

The input file is modified NEXUS format. A minimal example includes a tree (in newick format) and at least two dervived taxa set with the set derived command. If an outgroup is specified with set outgroup, the tree will be pruned to contain only taxa relevant to the simulation (i.e., the subclade containing derived taxa) + the outgroup.

#NEXUS
begin trees;
	tree tree_1 = (spA:0.002,(spB:0.001,((spC:0.0004,spD:0.0008)10.0:0.0005,(spE:0.0006,spF:0.0004)8.0:0.0004)15:0.0009)90.0:0.005);
end;

begin hemiplasytool;
set derived taxon=spB
set derived taxon=spD
set derived taxon=spF
end;

Species tree

Species tree in newick format. Branch lengths must be in average substitutions per site and branches must be labeled with concordance factors. IQTree can be used to do this.

If your tree is already ultrametric and in coalescent units, you can supply this directly if you add the flag set type coal to the input file.

Traits

Set which taxa have the derived character by using

set derived taxon="species in tree"

Introgression

Introgression events can be defined by using

set introgression source="species in tree" dest="species in tree" prob=[float_value] timing=[float_value]

Note that timing must be specified in coalescent units. For this reason, we recommend first running your input tree through subs2coal

Example:

python -m hemiplasytool -n 100000 -x 5 -p ~/msdir/ms -g ~/Seq-Gen-1.3.4/seq-gen -o test_w_introgression -v test/input_test_small_intro_v2.txt

Output:

Three output files will be produced. The main output test_w_introgression.txt, a gene trees file test_w_introgression.trees which contains all observed topologies, and a mutation distribution plot test_w_introgression.dist.png.

### INPUT SUMMARY ###

Integer Code	Taxon Name
1:	sp1
2:	sp2
3:	sp3
4:	sp4
5:	sp5
6:	sp6

The species tree (smoothed, in coalescent units) is:
 (1:2.78984,(2:2.09238,((3:0.69746,4:0.69746)1:0.69746,(5:0.69746,6:0.69746)1:0.69746)1:0.69746)1:0.69746);

  _________________________________ 1
 |
_|        _________________________ 2*
 |       |
 |_______|                 ________ 3
         |         _______|
         |        |       |________ 4*
         |________|
                  |        ________ 5
                  |_______|
                          |________ 6*

3 taxa have the derived state: 2, 4, 6

With homoplasy only, 3 mutations are required to explain this trait pattern (Fitch parsimony)

Introgression from taxon 4 into taxon 6 occurs at time 0.3 with probability 0.05

5.00e+05 simulations performed

### RESULTS ###

70 loci matched the species character states

"True" hemiplasy (1 mutation) occurs 14 time(s)

Combinations of hemiplasy and homoplasy (1 < # mutations < 3) occur 30 time(s)

"True" homoplasy (>= 3 mutations) occurs 26 time(s)

70 loci have a discordant gene tree
0 loci are concordant with the species tree

4 loci originate from an introgressed history
66 loci originate from the species history

Distribution of mutation counts:

# Mutations	# Trees
On all trees:
1		14
2		30
3		25
4		1

On concordant trees:
# Mutations	# Trees

On discordant trees:
# Mutations	# Trees
1		14
2		30
3		25
4		1

Origins of mutations leading to observed character states for hemiplasy + homoplasy cases:

	Tip mutation	Internal branch mutation	Tip reversal
Taxa 2	3	27	0
Taxa 4	3	27	0
Taxa 6	0	30	0

### OBSERVED GENE TREES ###

                 _________________ 4
  ______________|
 |              | ________________ 3
 |              ||
 |               |         _______ 5
_|               |________|
 |                        |_______ 6*
 |
 |    _____________________________ 1
 |___|
     |_____________________________ 2

This topology occured 1 time(s)
                       ____________ 3
  ____________________|
 |                    |____________ 5
_|
 |         ________________________ 1
 |________|
          |     __________________ 2
          |____|
               |        __________ 4
               |_______|
                       |__________ 6*

This topology occured 4 time(s)
         _________________________ 2
  ______|
 |      |       __________________ 5
 |      |______|
 |             |   _______________ 4
_|             |__|
 |                |_______________ 6*
 |
 |  _______________________________ 1
 |_|
   |_______________________________ 3

...

See test_w_introgression.txt.txt for full output.

Mutation distribution

Sub-modules

Once installed, two additional programs will be available at the command line: newick2ms and subs2coal.

newick2ms

usage: newick2ms [-h] input

Tool for converting a newick string to ms-style splits. Note that this only
makes sense if the input tree is in coalescent units.

positional arguments:
  input       Input newick string file

optional arguments:
  -h, --help  show this help message and exit

subs2coal

usage: subs2coal [-h] input

Tool for converting a newick string with branch lengths in subs/site to a
neewick string with branch lengths in coalescent units. Input requires gene or
site-concordancee factors as branch labels

positional arguments:
  input       Input newick string file

optional arguments:
  -h, --help  show this help message and exit

heistMerge

usage: heistmerge [-h] [-d] [inputs [inputs ...]]

Merge output files from multiple HeiST runs. Useful for simulating large trees
by running multiple batch jobs.

positional arguments:
  inputs      Prefixes of output files to merge or a directory (supply -d flag
              as well)

optional arguments:
  -h, --help  show this help message and exit
  -d          Merge all files in a directory

heistMerge will write the merged output summary to standard out and create a new files merged_trees.trees which contains all observed focal gene trees.

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