hs2p 2.1.0

Optimized slide tiling library for histopathology

hs2p

hs2p is a Python package for efficient slide tiling and tile sampling at any requested spacing, whether or not that spacing is natively present in the whole-slide image. It is designed for computational pathology workflows that need reproducible coordinates.

We support two main workflows:

• a Python API for library-style integration
• a CLI for batch preprocessing

Demo

Try hs2p interactively: hs2p-demo on HuggingFace Spaces
You can adjust tiling parameters (spacing, tile size, tissue threshold, overlap) and instantly see a tiling preview and tissue mask overlay.
You can also upload your own pyramidal WSI (up to 1 GB).

Installation

pip install hs2p

Workflows

Tiling

Tiling computes a reproducible grid of tile coordinates for each slide and saves them as named artifacts with extraction metadata, ready for downstream use.
When a precomputed tissue mask is not provided, hs2p segments tissue on-the-fly. If you want to precompute tissue masks, a standalone script is available.

Sampling

Sampling filters or partitions tile coordinates by annotation coverage so you can keep only tiles relevant to a tissue class or label.

Python API

hs2p supports pre-extracted tissue masks. If you don't have such tissue masks, you can either:

• use our standalone tissue segmentation script (Recommended)
• tune the SegmentationConfig parameters and let hs2p segments tissue on the fly

Minimal tiling example:

from pathlib import Path

from hs2p import (
    SlideSpec,
    TilingConfig,
    overlay_mask_on_slide,
    save_tiling_result,
    tile_slide,
    write_tiling_preview,
)

result = tile_slide(
    SlideSpec(
        sample_id="slide-1",
        image_path=Path("/data/wsi/slide-1.tif"),
        mask_path=Path("/data/mask/slide-1.tif"),
    ),
    tiling=TilingConfig(
        backend="openslide",
        target_spacing_um=0.5,
        target_tile_size_px=224,
        tolerance=0.07,
        overlap=0.0,
        tissue_threshold=0.1,
    ),
)

artifacts = save_tiling_result(result, output_dir=Path("output"))

print(artifacts.tiles_npz_path)   # output/coordinates/slide-1.tiles.npz ; more info in docs/artifacts.md
print(artifacts.tiles_meta_path)  # output/coordinates/slide-1.tiles.meta.json ; more info in docs/artifacts.md

tiling_preview_path = write_tiling_preview(
    result=result,
    output_dir=Path("output"),
    downsample=32,
)
print(tiling_preview_path)  # output/visualization/tiling/slide-1.jpg ; low resolution preview of tiling result, good for QC

mask_overlay = overlay_mask_on_slide(
    wsi_path=result.image_path,
    annotation_mask_path=Path("/data/mask/slide-1.tif"),
    downsample=32,
    backend=result.backend,
)
mask_overlay.save("output/visualization/mask/slide-1.jpg")

result is a TilingResult for one slide. It gives downstream pipelines the tile coordinates plus the metadata needed to relate those coordinates back to the slide pyramid and persist them as reusable named artifacts.

More API details: docs/api.md

CLI

The CLI is intended for fast batch processing of multiple slides with the same config. Both CLI entrypoints expect the same input csv schema:

sample_id,image_path,mask_path
slide-1,/data/wsi/slide-1.tif,/data/mask/slide-1.tif
slide-2,/data/wsi/slide-2.tif,

For a first run, start from hs2p/configs/default.yaml and edit only the essentials:

• csv
• output_dir
• tiling.backend
• tiling.params.target_spacing_um
• tiling.params.target_tile_size_px

Run tiling:

python -m hs2p.tiling --config-file /path/to/config.yaml

Run sampling:

python -m hs2p.sampling --config-file /path/to/config.yaml

For sampling, add tiling.sampling_params.pixel_mapping and tiling.sampling_params.tissue_percentage for the annotations you want to keep.

More CLI details: docs/cli.md

Outputs

hs2p writes explicit named artifacts rather than anonymous coordinate dumps.

• Tiling writes coordinates/{sample_id}.tiles.npz and coordinates/{sample_id}.tiles.meta.json
• Sampling writes the same pair under coordinates/<annotation>/
• Batch runs also write process_list.csv
• Saved coordinate arrays use a deterministic column-major order: numeric x first, then numeric y within each shared x

Artifact field reference: docs/artifacts.md

Docker

If you prefer running hs2p in a container, a published Docker image is available:

docker pull waticlems/hs2p:latest
docker run --rm -it -v /path/to/your/data:/data waticlems/hs2p:latest

Documentation

• Documentation index
• Python API guide
• CLI guide
• Artifact format reference
• Tissue mask generation script