Just a simple virus's hosts predictor
Project description
JASPER
JASPER is a free bioinformatics tools for predicting virus hosts.
JASPER uses a bunch of bioinformatics tools to prediction virus hosts. It includes genome-genome alignment, CRISPR spacers analyzation, tRNA analyzation and more.
JASPER contains few, independent modules blast
, crispr
, trna - not included now
, merge
.
Requirements
Naming convention
Jasper depends on good file naming convention. The best is to use sequence ID as file name, e.x. NC_008876.fna
. Software will use this id to name every temp file that needs to be created and also it will use this ID in results file.
WARNING It's not the best idea to use |
char in your filename and also in sequence header. Just use normal fasta naming like >NC_00876 additional_info more_additional_info
.
If you put multiple contigs in a single file, there is no problem with that. Just be sure that every contig is in it's right file. Jasper repairs every file it read, by default naming it <id from filename>|<#contig>
e.x.:
>NC_000856|1
ATGCT....
>NC_000856|2
ATGCA....
# and so on
So even if you have, for instance, one genome in your file, then Jasper will change it's id to <id from filename>|1
.
EXTENSIONS! Jasper uses input files that ends with (fa, fna, fasta) only!
Additional software
NCBI-Blast+
PILER-CR
tRNAscan-SE # not included in current release
Installation
I will make a simple installation script in my free time.
JASPER uses additional software. It calls every program with subprocess
so every program that is stated in above should be installed and added to $PATH
.
On Ubuntu:
- To install NCBI-Blast+ use
sudo apt install ncbi-blast+
- To install PILER-CR go here, dowload compiled software, move somwhere and add to
$PATH
under namepilercr
. - To install tRNAscan-SE use
sudo apt install trnascan-se
Source code for additional software:
Remember to install everything and add it to path
After that go to JASPER's main directory and:
python setup.py install
or you can use pip pip3 install jasper-vh
or python -m pip install jasper-vh
.
By defaults some pip on linux drops scripts to ~/local/bin
. Add it to your $PATH
at the end.
export PATH="$HOME/.local/bin:$PATH"
Now you're done and you can start using jasper-vh
.
Tests
If you want to test, go to proj directory and type python -m unittest discover
.
Usage
JASPER uses bunch of arguments. A lot of parameters are BLAST parameters and can be configured with JSON file and passed to JASPER.
Basic usage
jasper-vh blast --virus path/to/virus/dir --create-db host_db --host /path/to/host/dir
jasper-vh crispr --host path/to/host/dir --create-db vir_db --host /path/to/vir/dir
jasper-vh trna --args # soon
jasper-vh merge results1.csv results2.csv results3.csv [-w --weigths] 0.6 0.2 0.2 --output final_results.csv
For more check --help
on jasper individual modules: jasper-vh {blast,crispr,trna,merge} --help
Blast config
You can provide blast config as as a *.json
file.
Every module uses different task so there are few arguments that are forbidden:
[query', 'db', 'outfmt', 'max_target_seqs', 'num_alignments']
References
- Edgar, R.C. (2007) PILER-CR: fast and accurate identification of CRISPR repeats, BMC Bioinformatics, Jan 20;8:18
- Fichant and Burks, J. Mol. Biol. (1991) Identification of tRNA genes in genomic DNA, 220:659-671.
- NCBI-BLAST+
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