jcvi 1.5.4

Python utility libraries on genome assembly, annotation and comparative genomics

JCVI: A Versatile Toolkit for Comparative Genomics Analysis

Collection of Python libraries to parse bioinformatics files, or perform computation related to assembly, annotation, and comparative genomics.

Authors	Haibao Tang (tanghaibao)
	Vivek Krishnakumar (vivekkrish)
	Adam Taranto (Adamtaranto)
	Xingtan Zhang (tangerzhang)
	Won Cheol Yim (wyim-pgl)
Email	[e-mail]
License	BSD

How to cite

[!TIP] JCVI is now published in iMeta!

Tang et al. (2024) JCVI: A Versatile Toolkit for Comparative Genomics Analysis. iMeta

Contents

Following modules are available as generic Bioinformatics handling methods.

• algorithms

  • Linear programming solver with SCIP and GLPK.
  • Supermap: find set of non-overlapping anchors in BLAST or NUCMER output.
  • Longest or heaviest increasing subsequence.
  • Matrix operations.

• apps

  • GenBank entrez accession, Phytozome, Ensembl and SRA downloader.
  • Calculate (non)synonymous substitution rate between gene pairs.
  • Basic phylogenetic tree construction using PHYLIP, PhyML, or RAxML, and viualization.
  • Wrapper for BLAST+, LASTZ, LAST, BWA, BOWTIE2, CLC, CDHIT, CAP3, etc.

• formats

  Currently supports .ace format (phrap, cap3, etc.), .agp (goldenpath), .bed format, .blast output, .btab format, .coords format (nucmer output), .fasta format, .fastq format, .fpc format, .gff format, obo format (ontology), .psl format (UCSC blat, GMAP, etc.), .posmap format (Celera assembler output), .sam format (read mapping), .contig format (TIGR assembly format), etc.

• graphics

  • BLAST or synteny dot plot.
  • Histogram using R and ASCII art.
  • Paint regions on set of chromosomes.
  • Macro-synteny and micro-synteny plots.
  • Ribbon plots from whole genome alignments.

• utils

  • Grouper can be used as disjoint set data structure.
  • range contains common range operations, like overlap and chaining.
  • Miscellaneous cookbook recipes, iterators decorators, table utilities.

Then there are modules that contain domain-specific methods.

• assembly

  • K-mer histogram analysis.
  • Preparation and validation of tiling path for clone-based assemblies.
  • Scaffolding through ALLMAPS, optical map and genetic map.
  • Pre-assembly and post-assembly QC procedures.

• annotation

  • Training of ab initio gene predictors.
  • Calculate gene, exon and intron statistics.
  • Wrapper for PASA and EVM.
  • Launch multiple MAKER processes.

• compara

  • C-score based BLAST filter.
  • Synteny scan (de-novo) and lift over (find nearby anchors).
  • Ancestral genome reconstruction using Sankoff's and PAR method.
  • Ortholog and tandem gene duplicates finder.

Applications

Please visit wiki for full-fledged applications.

Dependencies

JCVI requires Python3 between v3.8 and v3.12.

Some graphics modules require the ImageMagick library.

On MacOS this can be installed using Conda (see next section). If you are using a linux system (i.e. Ubuntu) you can install ImageMagick using apt-get:

sudo apt-get update
sudo apt-get install libmagickwand-dev

See the Wand docs for instructions on installing ImageMagick on other systems.

A few modules may ask for locations of external programs, if the executable cannot be found in your PATH.

The external programs that are often used are:

• Kent tools
• BEDTOOLS
• EMBOSS

Managing dependencies with Conda

You can use the the YAML files in this repo to create an environment with basic JCVI dependencies.

If you are new to Conda, we recommend the Miniforge distribution.

conda env create -f environment.yml

conda activate jcvi

Note: If you are using a Mac with an ARM64 (Apple Silicon) processor, some dependencies are not currently available from Bioconda for this architecture.

You can instead create a virtual OSX64 (intel) env like this:

conda env create -f env_osx64.yml

conda activate jcvi-osx64

After activating the Conda environment install JCVI using one of the following options.

Installation

Installation options

1. Use pip to install the latest development version directly from this repo.

pip install git+git://github.com/tanghaibao/jcvi.git

2. Install latest release from PyPi.

pip install jcvi

3. Alternatively, if you want to install in development mode.

git clone git://github.com/tanghaibao/jcvi.git && cd jcvi
pip install -e '.[tests]'

Test Installation

If installed successfully, you can check the version with:

jcvi --version

Usage

Use python -m to call any of the modules installed with JCVI.

Most of the modules in this package contains multiple actions. To use the fasta example:

Usage:
    python -m jcvi.formats.fasta ACTION


Available ACTIONs:
          clean | Remove irregular chars in FASTA seqs
           diff | Check if two fasta records contain same information
        extract | Given fasta file and seq id, retrieve the sequence in fasta format
          fastq | Combine fasta and qual to create fastq file
         filter | Filter the records by size
         format | Trim accession id to the first space or switch id based on 2-column mapping file
        fromtab | Convert 2-column sequence file to FASTA format
           gaps | Print out a list of gap sizes within sequences
             gc | Plot G+C content distribution
      identical | Given 2 fasta files, find all exactly identical records
            ids | Generate a list of headers
           info | Run `sequence_info` on fasta files
          ispcr | Reformat paired primers into isPcr query format
           join | Concatenate a list of seqs and add gaps in between
     longestorf | Find longest orf for CDS fasta
           pair | Sort paired reads to .pairs, rest to .fragments
    pairinplace | Starting from fragment.fasta, find if adjacent records can form pairs
           pool | Pool a bunch of fastafiles together and add prefix
           qual | Generate dummy .qual file based on FASTA file
         random | Randomly take some records
         sequin | Generate a gapped fasta file for sequin submission
       simulate | Simulate random fasta file for testing
           some | Include or exclude a list of records (also performs on .qual file if available)
           sort | Sort the records by IDs, sizes, etc.
        summary | Report the real no of bases and N's in fasta files
           tidy | Normalize gap sizes and remove small components in fasta
      translate | Translate CDS to proteins
           trim | Given a cross_match screened fasta, trim the sequence
      trimsplit | Split sequences at lower-cased letters
           uniq | Remove records that are the same

Then you need to use one action, you can just do:

python -m jcvi.formats.fasta extract

This will tell you the options and arguments it expects.

Feel free to check out other scripts in the package, it is not just for FASTA.

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