Tool to find 3' tailing of non-coding RNAs
Project description
Tailer: A tool for 3' end analysis of non-polyadenylated RNAs from 3' sequencing data
Requirements
Python >= 3.6pysamBiogffuitlsrequeststqdm
Installation
pip install jla-tailer
Usage
Global alignment with a GTF annotation and SAM/BAM formatted files
Tailer -a [GTF Annotation] [SAM or BAM Files]
Local alignment with with FASTA/Q and specific Ensembl IDs of interest
Tailer -e [comma separated list of EnsIDs] [FASTA/Q files]
Optional arguments
-t, --threshold [int, default=100]- Any identified further than this distance in nucleotides from the mature end will be considered spurious and discarded
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