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Automatic ROI detection using cellpose, exports to .rgn files

Project description

Leica_ROI_finder

Automatic ROI finder for Leica Stellaris 8

  • Automatic segmentation using cellpose 3
  • ROI filtering using intensity, size and circularity
  • Exports ROIs to Leica .rgn files, can be imported into LAS X navigator

Installation

Leica ROI finder is available on PyPi and can be installed as follows:

pip install leica-roi-finder

Workflow

Load LIF image into ROI finder:

Perform segmentation using cellpose, filter ROIs using intensity, size and circularity:

Export ROIs to Leica .rgn format and import into LAS X Navigator:

Perform imaging on all regions:

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