Leica_ROI_finder
Automatic ROI finder for Leica Stellaris 8
- Automatic segmentation using cellpose 3
- ROI filtering using intensity, size and circularity
- Exports ROIs to Leica .rgn files, can be imported into LAS X navigator
Installation and usage
Leica ROI finder is available on PyPi and can be installed as follows:
pip install leica-scripts
Activate the GUI using:
python -m leica_scripts
Workflow
Load LIF image into ROI finder:
Perform segmentation using cellpose, filter ROIs using intensity, size and circularity:
Ron Scripts:
Perform imaging on all regions:
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