Generate interactive dotplot from MUMmer4 output using plotly
Project description
mummer-idotplot
Generate interactive dotplot from MUMmer4 output using plotly
Installation
$ pip install mummer-idotplot
plotly
is required to install.
Usage
When aligning two sequences with MUMmer4, you should set an option -F
(force 4 column output format) and -L
(show length of query). Also, you should not use -c
option, which change output position of match starting position and have bugs.
So for example:
If you have chr1, chr2
in reference.fasta
, and contig1, contig2
in query.fasta
$ mummer -maxmatch -F -L -b -l 10 reference.fasta query.fasta > output.mum
$ mummer-idotplot output.mum output.html --ref chr1 chr2 --query contig1 contig2
$ mummer-idotplot output.mum output.html --ref chr1:0:50000 chr2:100:20000 --query contig1 contig2 # you can specify the region to plot
$ open output.html # you'll see dotplot in your browser
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