nf-core 1.6

Helper tools for use with nf-core Nextflow pipelines.

Copyright (c) 2018 nf-core

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Description: # 

## [nf-core/tools](https://github.com/nf-core/tools)
[](https://travis-ci.org/nf-core/tools)
[](https://codecov.io/gh/nf-core/tools)
[](http://bioconda.github.io/recipes/nf-core/README.html)

A python package with helper tools for the nf-core community.

## Table of contents

* [`nf-core` tools installation](#installation)
* [`nf-core list` - List available pipelines](#listing-pipelines)
* [`nf-core launch` - Run a pipeline with interactive parameter prompts](#launch-a-pipeline)
* [`nf-core download` - Download pipeline for offline use](#downloading-pipelines-for-offline-use)
* [`nf-core licences` - List software licences in a pipeline](#pipeline-software-licences)
* [`nf-core create` - Create a new workflow from the nf-core template](#creating-a-new-workflow)
* [`nf-core lint` - Check pipeline code against nf-core guidelines](#linting-a-workflow)
* [`nf-core bump-version` - Update nf-core pipeline version number](#bumping-a-pipeline-version-number)


The nf-core tools package is written in Python and can be imported and used within other packages.
For documentation of the internal Python functions, please refer to the [Tools Python API docs](https://nf-co.re/tools-docs/).

## Installation

You can install `nf-core/tools` from [PyPI](https://pypi.python.org/pypi/nf-core/) using pip as follows:

```bash
pip install nf-core
```

Alternatively, it can be installed using [bioconda](https://bioconda.github.io/recipes/nf-core/README.html):

```bash
conda install -c bioconda nf-core
```

If you would like the development version instead, the command is:

```bash
pip install --upgrade --force-reinstall git+https://github.com/nf-core/tools.git
```

Alternatively, if you would like to edit the files locally:

```bash
# Clone the repository code - you should probably specify your fork instead
git clone https://github.com/nf-core/tools.git nf-core-tools
cd nf-core-tools

# Install the package
python setup.py develop

# Alternatively, install with pip
pip install -e .
```

## Listing pipelines
The command `nf-core list` shows all available nf-core pipelines along with their latest version, when that was published and how recently the pipeline code was pulled to your local system (if at all).

An example of the output from the command is as follows:

```console
$ nf-core list

,--./,-.
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|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'


Name Version Released Last Pulled Have latest release?
------------------------- --------- ------------ -------------- ----------------------
nf-core/rnaseq 1.3 4 days ago 27 minutes ago Yes
nf-core/hlatyping 1.1.4 3 weeks ago 1 months ago No
nf-core/eager 2.0.6 3 weeks ago - -
nf-core/mhcquant 1.2.6 3 weeks ago - -
nf-core/rnafusion 1.0 1 months ago - -
nf-core/methylseq 1.3 1 months ago 3 months ago No
nf-core/ampliseq 1.0.0 3 months ago - -
nf-core/deepvariant 1.0 4 months ago - -
nf-core/atacseq dev - 1 months ago No
nf-core/bacass dev - - -
nf-core/bcellmagic dev - - -
nf-core/chipseq dev - 1 months ago No
nf-core/clinvap dev - - -
```

To narrow down the list, supply one or more additional keywords to filter the pipelines based on matches in titles, descriptions and topics:

```console
$ nf-core list rna rna-seq

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|\ | |__ __ / ` / \ |__) |__ } {
| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'


Name Version Released Last Pulled Have latest release?
----------------- --------- ------------ -------------- ----------------------
nf-core/rnaseq 1.3 4 days ago 28 minutes ago Yes
nf-core/rnafusion 1.0 1 months ago - -
nf-core/lncpipe dev - - -
nf-core/smrnaseq dev - - -
```

You can sort the results by latest release (`-s release`, default),
when you last pulled a local copy (`-s pulled`),
alphabetically (`-s name`),
or number of GitHub stars (`-s stars`).

```console
$ nf-core list -s stars

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`._,._,'


Name Stargazers Version Released Last Pulled Have latest release?
------------------------- ------------ --------- ------------ -------------- ----------------------
nf-core/rnaseq 81 1.3 4 days ago 30 minutes ago Yes
nf-core/methylseq 22 1.3 1 months ago 3 months ago No
nf-core/ampliseq 21 1.0.0 3 months ago - -
nf-core/chipseq 20 dev - 1 months ago No
nf-core/deepvariant 15 1.0 4 months ago - -
nf-core/eager 14 2.0.6 3 weeks ago - -
nf-core/rnafusion 14 1.0 1 months ago - -
nf-core/lncpipe 9 dev - - -
nf-core/exoseq 8 dev - - -
nf-core/mag 8 dev - - -
```

Finally, to return machine-readable JSON output, use the `--json` flag.

## Launch a pipeline
Some nextflow pipelines have a considerable number of command line flags that can be used.
To help with this, the `nf-core launch` command uses an interactive command-line wizard tool to prompt you for
values for running nextflow and the pipeline parameters.

If the pipeline in question has a `parameters.settings.json` file following the [nf-core parameter JSON schema](https://nf-co.re/parameters.schema.json), parameters will be grouped and have associated description text and variable typing.

Nextflow `params` variables are saved in to a JSON file called `nfx-params.json` and used by nextflow with the `-params-file` flag.
This makes it easier to reuse these in the future.

It is not essential to run the pipeline - the wizard will ask you if you want to launch the command at the end.
If not, you finish with the `params` JSON file and a nextflow command that you can copy and paste.

```console
$ nf-core launch rnaseq

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`._,._,'


INFO: Launching nf-core/rnaseq
Main nextflow options

Config profile to use
-profile [standard]: docker

Unique name for this nextflow run
-name [None]: test_run

Work directory for intermediate files
-w [./work]:

Resume a previous workflow run
-resume [y/N]:

Release / revision to use
-r [None]: 1.3


Parameter group: Main options
Do you want to change the group's defaults? [y/N]: y

Input files
Specify the location of your input FastQ files.
--reads ['data/*{1,2}.fastq.gz']: '/path/to/reads_*{R1,R2}.fq.gz'

[..truncated..]

Nextflow command:
nextflow run nf-core/rnaseq -profile "docker" -name "test_run" -r "1.3" --params-file "/Users/ewels/testing/nfx-params.json"


Do you want to run this command now? [y/N]: y

INFO: Launching workflow!
N E X T F L O W ~ version 19.01.0
Launching `nf-core/rnaseq` [evil_engelbart] - revision: 37f260d360 [master]

[..truncated..]
```


## Downloading pipelines for offline use
Sometimes you may need to run an nf-core pipeline on a server or HPC system that has no internet connection. In this case you will need to fetch the pipeline files first, then manually transfer them to your system.

To make this process easier and ensure accurate retrieval of correctly versioned code and software containers, we have written a download helper tool. Simply specify the name of the nf-core pipeline and it will be downloaded to your current working directory.

By default, the pipeline will just download the pipeline code. If you specify the flag `--singularity`, it will also download any singularity image files that are required.

```console
$ nf-core download methylseq --singularity

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| \| | \__, \__/ | \ |___ \`-._,-`-,
`._,._,'


INFO: Saving methylseq
Pipeline release: 1.0
Pull singularity containers: Yes
Output directory: nf-core-methylseq-1.0

INFO: Downloading workflow files from GitHub

INFO: Downloading 1 singularity container
nf-core-methylseq-1.0.simg [762.28MB] [####################################] 780573/780572
```

```console
$ tree -L 2 nf-core-methylseq-1.0/

nf-core-methylseq-1.0/
├── singularity-images
│   └── nf-core-methylseq-1.0.simg
└── workflow
├── CHANGELOG.md
├── Dockerfile
├── LICENCE.md
├── README.md
├── assets
├── bin
├── conf
├── docs
├── environment.yml
├── main.nf
├── nextflow.config
└── tests

7 directories, 8 files
```

## Pipeline software licences
Sometimes it's useful to see the software licences of the tools used in a pipeline. You can use the `licences` subcommand to fetch and print the software licence from each conda / PyPI package used in an nf-core pipeline.

```console
$ nf-core licences rnaseq

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|\ | |__ __ / ` / \ |__) |__ } {
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`._,._,'


INFO: Warning: This tool only prints licence information for the software tools packaged using conda.
The pipeline may use other software and dependencies not described here.

Package Name Version Licence
--------------------- --------- --------------------
stringtie 1.3.3 Artistic License 2.0
preseq 2.0.3 GPL
trim-galore 0.4.5 GPL
bioconductor-edger 3.20.7 GPL >=2
fastqc 0.11.7 GPL >=3
openjdk 8.0.144 GPLv2
r-gplots 3.0.1 GPLv2
r-markdown 0.8 GPLv2
rseqc 2.6.4 GPLv2
bioconductor-dupradar 1.8.0 GPLv3
hisat2 2.1.0 GPLv3
multiqc 1.5 GPLv3
r-data.table 1.10.4 GPLv3
star 2.5.4a GPLv3
subread 1.6.1 GPLv3
picard 2.18.2 MIT
samtools 1.8 MIT
```

## Creating a new workflow
The `create` subcommand makes a new workflow using the nf-core base template.
With a given pipeline name, description and author, it makes a starter pipeline which follows nf-core best practices.

After creating the files, the command initialises the folder as a git repository and makes an initial commit. This first "vanilla" commit which is identical to the output from the templating tool is important, as it allows us to keep your pipeline in sync with the base template in the future.
See the [nf-core syncing docs](http://nf-co.re/sync) for more information.

```console
$ nf-core create

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Workflow Name: nextbigthing
Description: This pipeline analyses data from the next big 'omics technique
Author: Big Steve

INFO: Creating new nf-core pipeline: nf-core/nextbigthing

INFO: Initialising pipeline git repository

INFO: Done. Remember to add a remote and push to GitHub:
cd /path/to/nf-core-nextbigthing
git remote add origin [e-mail]:USERNAME/REPO_NAME.git
git push
```

Once you have run the command, create a new empty repository on GitHub under your username (not the `nf-core` organisation, yet).
On your computer, add this repository as a git remote and push to it:

```console
git remote add origin https://github.com/ewels/nf-core-nextbigthing.git
git push --set-upstream origin master
```

You can then continue to edit, commit and push normally as you build your pipeline.

Please see the [nf-core documentation](https://nf-co.re/adding_pipelines) for a full walkthrough of how to create a new nf-core workflow.

Note that if the required arguments for `nf-core create` are not given, it will interactively prompt for them. If you prefer, you can supply them as command line arguments. See `nf-core create --help` for more information.


## Linting a workflow
The `lint` subcommand checks a given pipeline for all nf-core community guidelines.
This is the same test that is used on the automated continuous integration tests.

For example, the current version looks something like this:

```console
$ cd path/to/my_pipeline
$ nf-core lint .

,--./,-.
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|\ | |__ __ / ` / \ |__) |__ } {
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`._,._,'

Running pipeline tests [####################################] 100% None

INFO: ===========
LINTING RESULTS
=================
72 tests passed 2 tests had warnings 0 tests failed

WARNING: Test Warnings:
http://nf-co.re/errors#8: Conda package is not latest available: picard=2.18.2, 2.18.6 available
http://nf-co.re/errors#8: Conda package is not latest available: bwameth=0.2.0, 0.2.1 available
```

You can find extensive documentation about each of the lint tests in the [lint errors documentation](https://nf-co.re/errors).


## Bumping a pipeline version number

When releasing a new version of a nf-core pipeline, version numbers have to be updated in several different places. The helper command `nf-core bump-version` automates this for you to avoid manual errors (and frustration!).

The command uses results from the linting process, so will only work with workflows that pass these tests.

Usage is `nf-core bump-version <pipeline_dir> <new_version>`, eg:

```console
$ cd path/to/my_pipeline
$ nf-core bump-version . 1.0

,--./,-.
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|\ | |__ __ / ` / \ |__) |__ } {
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`._,._,'


INFO: Running nf-core lint tests
Running pipeline tests [####################################] 100% None

INFO: ===========
LINTING RESULTS
=================
96 tests passed 0 tests had warnings 0 tests failed

INFO: Changing version number:
Current version number is '1.0dev'
New version number will be '1.0'

INFO: Updating version in nextflow.config
- version = '1.0dev'
+ version = '1.0'

INFO: Updating version in nextflow.config
- container = 'nfcore/mypipeline:dev'
+ container = 'nfcore/mypipeline:1.0'

INFO: Updating version in .travis.yml
- docker tag nfcore/mypipeline:dev nfcore/mypipeline:latest
+ docker tag nfcore/mypipeline:dev nfcore/mypipeline:1.0

INFO: Updating version in Singularity
- VERSION 1.0dev
+ VERSION 1.0

INFO: Updating version in environment.yml
- name: nf-core-mypipeline-1.0dev
+ name: nf-core-mypipeline-1.0

INFO: Updating version in Dockerfile
- PATH /opt/conda/envs/nf-core-mypipeline-1.0dev/bin:$PATH
+ PATH /opt/conda/envs/nf-core-mypipeline-1.0/bin:\$PATH

INFO: Updating version in Singularity
- PATH=/opt/conda/envs/nf-core-mypipeline-1.0dev/bin:$PATH
+ PATH=/opt/conda/envs/nf-core-mypipeline-1.0/bin:\$PATH
```

To change the required version of Nextflow instead of the pipeline version number, use the flag `--nextflow`.

Keywords: nf-core,nextflow,bioinformatics,workflow,pipeline,biology,sequencing,NGS,next generation sequencing
Platform: UNKNOWN
Description-Content-Type: text/markdown